Neuritin对大鼠蛛网膜下腔出血后早期脑损伤影响的研究

发布时间：2018-10-29 09:11

【摘要】：目的探究Neuritin对大鼠蛛网膜下腔出血(SAH)后早期脑损伤过程中内质网应激因子GRP78及血-脑脊液屏障通透性的影响。方法 2014年4月—2015年9月选取成年健康雄性SD大鼠144只,按照随机数字表法分为对照组、假手术组(Sham组)、SAH组、Neuritin组,各36只。再依据简单随机抽样法分别于3、6、12、24、48、72 h抽取每组6只大鼠处死,Neuritin组于处死前1 h采用立体定向技术向侧脑室注射Neuritin。建模后6、12、24、48、72 h处死大鼠前,记录各组大鼠Garcia神经功能评分;建模后3、6、12、24、48、72 h处死大鼠后,采用HE染色观察SAH建立情况,侧脑室微量注射亚甲蓝鉴定Neuritin注射情况;采用Western blotting法检测大鼠大脑皮质内质网应激因子GRP78表达水平;采用甲酰胺浸泡法检测大鼠脑内伊文蓝(EB)含量以评价血-脑脊液屏障通透性。结果 SAH组、Neuritin组建模后6、12、24、48、72 h大鼠Garcia神经功能评分均低于对照组和Sham组(P0.05);Neuritin组建模后6、12、24、48、72 h大鼠Garcia神经功能评分均高于SAH组(P0.05)。SAH组大鼠大脑表面弥散分布积血,其中在willis环、小脑延髓池及脑干腹侧有大量积血存在。Neuritin组大脑腹面有亚甲蓝循环存在,大脑背面无亚甲蓝,Neuritin侧脑室注射成功。SAH组建模后3、6、12、24、48、72 h GRP78表达水平高于对照组、Sham组(P0.05);Neuritin组建模后6、12、24、48、72 h GRP78表达水平低于SAH组(P0.05)。SAH组大鼠建模后3、6、12、24、48、72 h脑组织EB含量均高于对照组、Sham组(P0.05);Neuritin组大鼠建模后3、12 h脑组织EB含量高于SAH组,建模后6、48、72 h脑组织EB含量低于SAH组(P0.05)。结论 SAH后早期脑损伤过程中存在内质网应激反应,神经营养因子Neuritin可显著改变内质网应激因子GRP78表达水平,改善早期脑损伤过程中大鼠的神经行为学评分,并对血-脑脊液屏障通透性产生影响。
[Abstract]:Objective to investigate the effects of Neuritin on endoplasmic reticulum stress factor (GRP78) and blood-cerebrospinal fluid barrier permeability during early brain injury after (SAH) in rats with subarachnoid hemorrhage. Methods 144 healthy adult male SD rats were selected from April 2014 to September 2015. They were randomly divided into control group (Sham group,), SAH group, Neuritin group, 36 rats). According to the simple random sampling method, 6 rats in each group were killed in each group at 3h, 6h, 12h, 24h, 4872 h, respectively. In Neuritin group, Neuritin. was injected into lateral ventricle by stereotactic technique 1 hour before death. The Garcia neurological function scores were recorded before the rats were killed at 6: 12, 24, 48 and 72 hours after modeling, the establishment of SAH was observed by HE staining, and the injection of Neuritin was evaluated by microinjection of methylene blue into the lateral ventricle after the rats were killed at 3: 6, 12, 24, 48, 72 hours after modeling. Western blotting method was used to detect the expression of ER stress factor GRP78 and formamide immersion method was used to detect the content of Yi Wen blue (EB) in rat brain to evaluate the permeability of blood-cerebrospinal fluid barrier. Results the Garcia neurological function scores in SAH group and Neuritin group were lower than those in control group and Sham group (P0.05). The neurological function scores of Garcia in the Neuritin group were higher than those in the SAH group (P0.05) in the). SAH group, especially in the willis ring. In the Neuritin group, there was methylene blue circulation on the ventral surface of the brain, no methylene blue on the back of the brain and successful injection of Neuritin into the ventral ventricle. The expression of GRP78 in the SAH group was higher than that in the control group at 72h after modeling. Sham group (P0.05); The expression level of GRP78 in Neuritin group was lower than that in SAH group at 72 h after modeling (P0.05). The content of EB in brain tissue in). SAH group was significantly higher than that in control group (P0.05). The content of EB in brain tissue in Neuritin group was higher than that in SAH group at 12 hours after modeling, and the content of EB in brain tissue was lower than that in SAH group at 6 ~ 872 h after modeling (P0.05). Conclusion there is endoplasmic reticulum stress response during early brain injury after SAH. Neurotrophic factor Neuritin can significantly change the expression level of endoplasmic reticulum stress factor GRP78 and improve the neurobehavioral score of rats during early brain injury. The permeability of blood-cerebrospinal fluid barrier was also affected.
【作者单位】：石河子大学医学院第一附属医院神经外科;潍坊市人民医院;
【基金】：国家自然科学资金资助项目(81360185)
【分类号】：R743.35

【相似文献】