胰岛素样生长因子-1对缺氧缺血性脑损伤新生小鼠脑细胞线粒体细胞色素C、Caspase-3蛋白和mRNA表达的影响

发布时间：2018-11-01 16:57

【摘要】：目的探讨胰岛素样生长因子-1（insulin-like growth factor-1, IGF-1）对缺氧缺血性脑损伤(hypoxic-ischemic brain damage, HIBD)新生小鼠脑组织线粒体Cyt-C（细胞色素C）、caspase-3（半胱氨酸天冬氨酸蛋白酶-3）表达的影响。方法108只新生7日龄C57/BL小鼠随机分为正常对照组、缺氧缺血组（HI）组、HI+IGF-1组。采用改良Rice方法制备新生小鼠右脑HIBD动物模型，HI+IGF-1组于HI后即刻给予腹腔注射IGF-1（50μg/Kg），HI组和正常对照组注射等体积生理盐水。应用RT-PCR和Western Blot检测各组皮质、丘脑、海马0h、3h、6h、12h、24h及48h Cyt-C、caspase-3mRNA和蛋白的表达。。结果： ①HI组Cyt-C、caspase-3mRNA及蛋白表达明显增加出现在皮质术后3h，海马术后6h，丘脑出现在术后12h，明显迟于皮质及海马；②H I+IGF-1组Cyt-C、caspase-3mRNA及蛋白表达在HI组表达明显增加的相应时间点虽较正常对照组升高，但较HI组表达明显降低，三组之间比较差异有统计学意义（P0.05）；③H I组皮质Cyt-C、caspase-3mRNA及蛋白表达于术后3h表达明显增加，6h有所下降，，12-24h达到另一高峰，峰值时间点HI+IGF-1组的表达较HI组均降低（P0.05）。结论IGF-1可能通过抑制Cyt-C释放，减少caspase-3表达，发挥对新生小鼠HIBD的神经保护作用，IGF-1对皮质的保护有两个时间点。
[Abstract]:Objective to investigate the effect of insulin-like growth factor-1 (insulin-like growth factor-1, IGF-1) on mitochondrial Cyt-C (cytochrome C),) in brain tissue of hypoxic-ischemic brain injury (hypoxic-ischemic brain damage, HIBD) mice. Effect of caspase-3 (cysteine aspartate protease-3) expression. Methods 108 7 day old C57/BL mice were randomly divided into normal control group, (HI) group and HI IGF-1 group. The right brain HIBD model of newborn mice was established by modified Rice method. HI IGF-1 group was injected intraperitoneally with IGF-1 (50 渭 g/Kg), HI group and normal control group) immediately after HI. RT-PCR and Western Blot were used to detect the expression of Cyt-C,caspase-3mRNA and protein in cortex, thalamus and hippocampus. Results: the expression of Cyt-C,caspase-3mRNA and protein increased significantly in 1HI group at 3 hours after cortical operation, 6 hours after hippocampus operation and 12 hours after operation of thalamus, which was significantly later than that of cortex and hippocampus. 2The expression of Cyt-C,caspase-3mRNA and protein in the HI group was significantly higher than that in the normal control group, but lower than that in the HI group, and the difference between the three groups was statistically significant (P0.05). In 3H I group, the expression of Cyt-C,caspase-3mRNA and protein increased significantly at 3 h after operation, decreased at 6 h, reached another peak from 12 to 24 h, and the expression of HI IGF-1 at the peak time was lower than that in HI group (P0.05). Conclusion IGF-1 may play a neuroprotective role on HIBD in newborn mice by inhibiting the release of Cyt-C and decreasing the expression of caspase-3. IGF-1 has two time points for the protection of cortex.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R742

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