TRPC3在海人酸诱导的癫痫发作大鼠大脑皮质中表达上调
发布时间：2018-11-06 13:44
【摘要】：目的研究瞬时受体电位通道蛋白3(transient receptor potential canonical 3,TRPC3)在海人酸(kainic acid,KA)致痫大鼠大脑皮质中的表达变化,探讨其与癫痫发病的关系及意义。方法选取54只成年雄性SD大鼠随机分为对照组(NS组)18只和实验组(KA组)36只,实验组给予侧脑室注射KA 2μg/kg(7μL左右)制作KA致痫模型,对照组给予侧脑室注射等量生理盐水,观察记录大鼠癫痫发作的行为学表现。于癫痫发作后不同时间点(48h、72h)各取18只大鼠注射侧皮层脑组织,对照组于注射结束后48h取18只大鼠同侧皮层脑组织,用半定量RT-PCR、Western blot和免疫组织化学技术(均各6只)检测TRPC3的mRNA和蛋白的含量。结果对照组大鼠无癫痫发作,实验组大鼠于注射后5min左右出现典型痫性发作,达Ⅳ~Ⅴ级,持续数小时;实验组TRPC3的mRNA和蛋白表达水平在注射后48h和72h均较对照组显著增高(P0.05),但实验组两个时间点之间差异无统计学意义。结论 TRPC3在致痫大鼠大脑皮质中的表达增加,其可能参与了癫痫的形成和发展。
[Abstract]:Objective to investigate the expression of transient receptor channel protein 3 (transient receptor potential canonical 3 (TRPC3) in the cerebral cortex of epileptic rats induced by kainic acid (kainic acid,KA) and its relationship with epilepsy. Methods Fifty-four adult male SD rats were randomly divided into control group (NS group, n = 18) and experimental group (KA group, n = 36). The experimental group was given intraventricular injection of KA 2 渭 g/kg (about 7 渭 L) to make the model of KA induced epilepsy. Rats in the control group were given the same amount of saline to observe the behavior of epileptic seizures. Eighteen rats were injected with lateral cortical brain tissue at different time points (48h / 72h) after seizure, and 18 rats' ipsilateral cortical brain tissue were taken from control group at 48h after injection. Semi-quantitative RT-PCR, was used. The contents of mRNA and protein in TRPC3 were detected by Western blot and immunohistochemistry. Results there was no epileptic seizure in the control group, and typical epileptic seizure occurred in the experimental group after injection of 5min, reaching grade 鈪，
本文编号：2314447
[Abstract]:Objective to investigate the expression of transient receptor channel protein 3 (transient receptor potential canonical 3 (TRPC3) in the cerebral cortex of epileptic rats induced by kainic acid (kainic acid,KA) and its relationship with epilepsy. Methods Fifty-four adult male SD rats were randomly divided into control group (NS group, n = 18) and experimental group (KA group, n = 36). The experimental group was given intraventricular injection of KA 2 渭 g/kg (about 7 渭 L) to make the model of KA induced epilepsy. Rats in the control group were given the same amount of saline to observe the behavior of epileptic seizures. Eighteen rats were injected with lateral cortical brain tissue at different time points (48h / 72h) after seizure, and 18 rats' ipsilateral cortical brain tissue were taken from control group at 48h after injection. Semi-quantitative RT-PCR, was used. The contents of mRNA and protein in TRPC3 were detected by Western blot and immunohistochemistry. Results there was no epileptic seizure in the control group, and typical epileptic seizure occurred in the experimental group after injection of 5min, reaching grade 鈪，
本文编号：2314447
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