大黄酚对局灶性脑缺血再灌注小鼠缺血半暗带区环氧化酶2和基质金属蛋白酶-9表达的影响
发布时间：2018-11-07 12:10
【摘要】：目的 探究大黄酚(Chrysophanol,CHR)对大脑中动脉梗死(middle cerebral artery occlusion,MCAO)模型小鼠再灌注后脑内环氧化酶2(cyclooxygenase-2,COX2)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)表达的影响,探讨CHR保护脑缺血再灌注损伤的抗炎机制。方法 采用数字表法随机将18只健康雄性C57BL小鼠分为3组:假手术(Sham)组、MCAO组、CHR组(小鼠造模当天按0.1 mg/kg腹腔注射CHR,此后每天1次,连续给药14 d),每组6只。使用线栓法制作小鼠右侧大脑中动脉缺血45 min再灌注模型。术中监测小鼠肛温,使其维持在正常范围。于再灌注后14 d处死小鼠,迅速取脑,用免疫荧光染色检测小鼠脑组织冰冻切片缺血半暗带区COX2和MMP-9的表达,并用免疫荧光双标法对COX2和MMP-9在缺血脑组织的表达进行细胞定位。结果 1)Sham组小鼠偶见COX2或MMP-9染色阳性细胞。与Sham组相比,MCAO组小鼠脑缺血半暗带区COX2和MMP-9的表达明显增高(P0.05)。2)与MCAO组相比,给予CHR治疗后,缺血再灌注小鼠脑缺血半暗带区COX2和MMP-9的表达均明显减少(P0.05)。3)缺血再灌注小鼠脑缺血半暗带区,COX2或MMP-9免疫荧光染色分别与神经元标志物Neu N免疫荧光染色共定位。结论 CHR可能通过抑制COX2和MMP-9的蛋白表达,减轻炎性反应,从而对脑缺血再灌注损伤发挥长期的神经保护作用。
[Abstract]:Objective to investigate the effects of chrysophanol (Chrysophanol,CHR) on the expression of cyclooxygenase-2 (cyclooxygenase-2,COX2) and matrix metalloproteinase-9 (matrix metalloproteinase-9,MMP-9) after reperfusion in (middle cerebral artery occlusion,MCAO model mice with middle cerebral artery infarction. To investigate the anti-inflammatory mechanism of CHR in the protection of cerebral ischemia reperfusion injury. Methods 18 healthy male C57BL mice were randomly divided into 3 groups: sham operation (Sham) group, MCAO group and CHR group (mice were injected with CHR, once a day according to 0.1 mg/kg per day after CHR, was injected intraperitoneally for 14 d),). There were 6 rats in each group. The right middle cerebral artery (MCAA) ischemia reperfusion model of mice was established with thread occlusion for 45 min. The anus temperature of mice was monitored during operation to keep it in normal range. The mice were killed 14 days after reperfusion. The expression of COX2 and MMP-9 in the ischemic penumbra of frozen sections of the brain were detected by immunofluorescence staining. The expression of COX2 and MMP-9 in ischemic brain tissue was detected by immunofluorescence double labeling method. Results 1) COX2 or MMP-9 positive cells were occasionally found in Sham group mice. Compared with Sham group, the expression of COX2 and MMP-9 in cerebral ischemic penumbra in MCAO group was significantly higher than that in Sham group (P0.05). Compared with MCAO group, the expression of COX2 and MMP-9 in MCAO group was significantly higher than that in MCAO group. The expression of COX2 and MMP-9 in cerebral ischemic penumbra decreased significantly in ischemia-reperfusion mice (P0.05). COX2 or MMP-9 immunofluorescence staining were co-located with Neu N immunofluorescence staining. Conclusion CHR may play a long-term neuroprotective role in cerebral ischemia-reperfusion injury by inhibiting the expression of COX2 and MMP-9 proteins and reducing inflammatory response.
【作者单位】： 首都医科大学宣武医院北京市老年病医疗研究中心神经变性病教育部重点实验室脑血管病转化医学北京市重点实验室;
【分类号】：R743.3
本文编号：2316331
[Abstract]:Objective to investigate the effects of chrysophanol (Chrysophanol,CHR) on the expression of cyclooxygenase-2 (cyclooxygenase-2,COX2) and matrix metalloproteinase-9 (matrix metalloproteinase-9,MMP-9) after reperfusion in (middle cerebral artery occlusion,MCAO model mice with middle cerebral artery infarction. To investigate the anti-inflammatory mechanism of CHR in the protection of cerebral ischemia reperfusion injury. Methods 18 healthy male C57BL mice were randomly divided into 3 groups: sham operation (Sham) group, MCAO group and CHR group (mice were injected with CHR, once a day according to 0.1 mg/kg per day after CHR, was injected intraperitoneally for 14 d),). There were 6 rats in each group. The right middle cerebral artery (MCAA) ischemia reperfusion model of mice was established with thread occlusion for 45 min. The anus temperature of mice was monitored during operation to keep it in normal range. The mice were killed 14 days after reperfusion. The expression of COX2 and MMP-9 in the ischemic penumbra of frozen sections of the brain were detected by immunofluorescence staining. The expression of COX2 and MMP-9 in ischemic brain tissue was detected by immunofluorescence double labeling method. Results 1) COX2 or MMP-9 positive cells were occasionally found in Sham group mice. Compared with Sham group, the expression of COX2 and MMP-9 in cerebral ischemic penumbra in MCAO group was significantly higher than that in Sham group (P0.05). Compared with MCAO group, the expression of COX2 and MMP-9 in MCAO group was significantly higher than that in MCAO group. The expression of COX2 and MMP-9 in cerebral ischemic penumbra decreased significantly in ischemia-reperfusion mice (P0.05). COX2 or MMP-9 immunofluorescence staining were co-located with Neu N immunofluorescence staining. Conclusion CHR may play a long-term neuroprotective role in cerebral ischemia-reperfusion injury by inhibiting the expression of COX2 and MMP-9 proteins and reducing inflammatory response.
【作者单位】： 首都医科大学宣武医院北京市老年病医疗研究中心神经变性病教育部重点实验室脑血管病转化医学北京市重点实验室;
【分类号】：R743.3
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