抗N-甲基-D-天冬氨酸受体脑炎患者血浆microRNA-140表达的初步研究

发布时间：2018-11-18 19:18

【摘要】：目的探讨抗N-甲基-D-天冬氨酸受体(NMDAR)脑炎患者血浆中microRNA-140表达变化及其意义。方法应用微阵列基因芯片技术检测抗NMDAR脑炎患者和健康人血浆microRNAs的表达水平;通过实时定量PCR方法检测5例抗NMDAR脑炎患者(实验组)、20例结核性脑(膜)炎患者(结脑组)、20例病毒性脑(膜)炎患者(病脑组)、30例健康人(对照组)血浆microRNA-140的表达水平。结果 (1)基因芯片表明血浆microRNA-140的表达水平在抗NMDAR脑炎患者中显著低于健康人,差异具有统计学意义(log2=5.11,P=0.004);通过实时定量PCR方法验证,与芯片检测结果一致,差异具有统计学意义(P0.05);(2)血浆microRNA-140的表达水平在实验组中显著低于结脑组、病脑组、对照组(P0.05);结脑组、病脑组与对照组相比,血浆中microRNA-140水平下调差异无统计学意义(P0.05)。结论抗NMDAR脑炎患者血浆中microRNA-140水平明显下调,提示microRNA-140可能参与了抗NMDAR脑炎的发生、发展过程。
[Abstract]:Objective to investigate the changes and significance of microRNA-140 expression in plasma of patients with anti-N-methyl-d-aspartic acid receptor (NMDAR) encephalitis. Methods the expression of microRNAs in plasma of patients with NMDAR encephalitis and healthy subjects was detected by microarray gene chip technique. Real-time quantitative PCR was used to detect 5 patients with anti NMDAR encephalitis (experimental group), 20 patients with tuberculous meningitis (tuberculous meningetis) and 20 patients with viral meningetis (disease brain group). Expression of microRNA-140 in 30 healthy subjects (control group). Results (1) the expression of microRNA-140 in plasma was significantly lower in patients with anti NMDAR encephalitis than that in healthy controls (log2=5.11,P=0.004). The results of real-time quantitative PCR were consistent with the results of microarray detection. The difference was statistically significant (P0.05); (2) the expression of plasma microRNA-140 in the experimental group was significantly lower than that in the nodular brain group disease brain group and control group (P0.05). There was no significant difference in the down-regulation of plasma microRNA-140 levels between the nodule group and the diseased brain group compared with the control group (P0.05). Conclusion the level of plasma microRNA-140 in patients with anti NMDAR encephalitis is down-regulated, suggesting that microRNA-140 may be involved in the occurrence and development of anti NMDAR encephalitis.
【作者单位】：郑州大学第一附属医院神经内科;
【基金】：国家自然科学基金项目(No.81071114)
【分类号】：R742.9

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