颞叶癫痫microRNA基因甲基化模式分析及机制探究
发布时间：2018-11-18 21:35
【摘要】：目的重注释甲基化数据并构建microRNA(miRNA)基因甲基化谱,探究差异甲基化miRNA在颞叶癫痫(TLE)发生发展及耐药机制中的作用。方法收集TLE患者以及健康对照外周血,提取DNA进行全基因组DNA甲基化检测。将甲基化数据重注释至miRNA基因,统计分析筛选病例组与对照组以及临床亚组之间的差异甲基化miRNA,运用生物信息学方法对差异甲基化miRNA功能分析。结果 TLE和对照组间有82个miRNA基因甲基化存在差异(FDR5%),其中甲基化升高的70个。临床亚组间也存在差异甲基化miRNA基因(P0.01)。差异甲基化miRNA基因参与MAPK信号通路、神经营养信号通路等多条生物学通路。结论 TLE患者外周血miRNA基因组甲基化存在异常,以甲基化程度升高为主。差异甲基化miRNA基因参与多条生物学通路,可能在TLE发病及耐药机制中起到重要作用。
[Abstract]:Objective to reinterpret the methylation data and construct the methylation spectrum of microRNA (miRNA) gene to explore the role of differential methylation miRNA in the pathogenesis and drug resistance of (TLE) in temporal lobe epilepsy. Methods Peripheral blood samples from patients with TLE and healthy controls were collected and DNA was extracted for genomic DNA methylation detection. The methylation data were reannotated to miRNA gene, and the differential methylation miRNA, was selected between the case group, control group and clinical subgroup. The function of differential methylation miRNA was analyzed by bioinformatics. Results there were 82 miRNA gene methylation differences (FDR5%) between TLE and control group, 70 of them had higher methylation. There were also differences between clinical subgroups in methylation of miRNA gene (P0.01). Differentially methylated miRNA gene is involved in many biological pathways, such as MAPK signaling pathway and neurotrophic signaling pathway. Conclusion miRNA genomic methylation in peripheral blood of patients with TLE is abnormal and the degree of methylation is increased. Differential methylation of miRNA gene may play an important role in the pathogenesis and drug resistance of TLE.
【作者单位】： 中南大学湘雅医院神经内科;
【基金】：国家自然科学基金项目(81371435;81671299;81401078) 国家科技部重大研究专项《基于组学特征谱的癫痫分子分型研究》(2016YFC0904400)
【分类号】：R742.1
[Abstract]:Objective to reinterpret the methylation data and construct the methylation spectrum of microRNA (miRNA) gene to explore the role of differential methylation miRNA in the pathogenesis and drug resistance of (TLE) in temporal lobe epilepsy. Methods Peripheral blood samples from patients with TLE and healthy controls were collected and DNA was extracted for genomic DNA methylation detection. The methylation data were reannotated to miRNA gene, and the differential methylation miRNA, was selected between the case group, control group and clinical subgroup. The function of differential methylation miRNA was analyzed by bioinformatics. Results there were 82 miRNA gene methylation differences (FDR5%) between TLE and control group, 70 of them had higher methylation. There were also differences between clinical subgroups in methylation of miRNA gene (P0.01). Differentially methylated miRNA gene is involved in many biological pathways, such as MAPK signaling pathway and neurotrophic signaling pathway. Conclusion miRNA genomic methylation in peripheral blood of patients with TLE is abnormal and the degree of methylation is increased. Differential methylation of miRNA gene may play an important role in the pathogenesis and drug resistance of TLE.
【作者单位】： 中南大学湘雅医院神经内科;
【基金】：国家自然科学基金项目(81371435;81671299;81401078) 国家科技部重大研究专项《基于组学特征谱的癫痫分子分型研究》(2016YFC0904400)
【分类号】：R742.1
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