磁敏感加权成像对高血压大鼠模型脑微出血的实验研究
发布时间：2018-12-08 09:16
【摘要】：目的： 探讨磁敏感加权图像在检出脑微出血与病理的关系。探讨ESWAN的R2STAR值与脑发生微出血的相关关系。明确MRI-ESWAN扫描皮层及基底节的R2STAR值的骤然升高是否可以提前1-2周预示脑微出血。 材料与方法： 获得卒中易感性自发性高血压（SHR）高龄雄性鼠模型30只，按照实验计划连续监测血压及定期核磁共振扫描。使用血压仪监测时记录每只SHR鼠的收缩压（SBP）及舒张压（DBP）。使用动物线圈扫描，MRI扫描序列包括ESWAN、3D-FRSG及T2-WI，测量ESWAN中的R2STAR值，并且观察相位图和/或大鼠脑内是否出现微出血灶，记录微出血灶的时间及部位。ESWAN序列相位图和/或幅值图脑内任何部位微出血或出血灶为阳性组，未发现微出血或出血灶为阴性组。ESWAN发现脑出血后处死大鼠，进行脑组织病理切片观察并与MRI图像对比分析。未出血组在实验终点处死。采用重复测量方差分析比较出血前多个不同时间、不同部位的R2STAR值。 结果： 卒中易感性自发性高血压（SHR）高龄雄性鼠模型17只出现自发性脑出血，9只未出血，4只在实验中死亡。阳性组与阴性组的血压之间无统计学意义（P0.05）。阳性组出血前1-2W大脑皮层、基底节区R2STAR值病变侧中心区高于阴性组末次，有统计学差异（P0.05)。38-68W阳性组病变侧中心区及对侧镜相区R2STAR值均平稳上升，68-74W阳性组病变侧中心区R2STAR值上升较快（P0.05)，对侧镜相区变化相对平缓。阳性组出血前1-2W大脑皮层、基底节区R2STAR值病变侧中心区明显高于对侧镜相区，，有统计学差异（P0.05)。后处理ESWAN图像中微出血灶或出血灶分布与病理切片观察分布基本一致。ESWAN的R2STAR值升高的部位与随后出现脑微出血的部位基本一致。 结论： 磁敏感加权图像能够检测出脑微出血灶，基本准确的反映微血管病变的病理改变；皮层及基底节的R2STAR值升高的部位相继发生微出血，且出血的位置与病理基本一致。采用ESWAN扫描，测量R2STAR值的变化可能预示高血压自发性脑内出血；
[Abstract]:Objective: to study the relationship between magnetic sensitivity weighted image and pathology in detecting cerebral microhemorrhage. To investigate the correlation between R2STAR value of ESWAN and cerebral microhemorrhage. To determine whether the sudden increase of R2STAR in cortical and basal ganglia on MRI-ESWAN scan can predict cerebral microhemorrhage 1-2 weeks ahead of time. Materials and methods: thirty aged male (SHR) rats with stroke susceptibility to spontaneous hypertension were obtained and their blood pressure was continuously monitored and MRI scans were performed regularly according to the experimental plan. Systolic blood pressure (SBP) and diastolic blood pressure (DBP).) were recorded in each SHR rat using a blood pressure monitor Using animal coil scanning and MRI scanning sequences including ESWAN,3D-FRSG and T2-WI, the R2STAR values in ESWAN were measured, and the phase map and / or microbleeds were observed in the brain. The time and location of microhemorrhage were recorded. ESWAN sequence phase map and / or amplitude map showed that microhemorrhage or haemorrhage was positive in any part of the brain, but no microhemorrhage or haemorrhage was found in negative group. The rats were killed after ESWAN found intracerebral hemorrhage. The pathological sections of brain tissue were observed and compared with MRI images. No bleeding group was killed at the end of the experiment. Repeated measurement variance analysis was used to compare the R2STAR values of different parts at different time before bleeding. Results: there were 17 cases of spontaneous cerebral hemorrhage, 9 cases of no hemorrhage and 4 cases of death in the male model of stroke susceptible spontaneous hypertension (SHR). There was no significant difference in blood pressure between positive group and negative group (P0.05). The R2STAR values of cortex and basal ganglia in the positive group were higher than those in the negative group (P0.05). The R2STAR values of the lesion side and the contralateral area in the 38-68W positive group were increased steadily. In the 68-74W positive group, the R2STAR value in the central region of the lesion increased rapidly (P0.05), and the change of the contralateral mirror phase was relatively gentle. In the positive group, the R2STAR values of cerebral cortex and basal ganglia were significantly higher than those of the contralateral area before hemorrhage (P0.05). The distribution of microhaemorrhage or haemorrhage in post-processing ESWAN images was basically the same as that observed by pathological sections, and the location where the R2STAR value of ESWAN increased was basically the same as that of subsequent intracerebral microhaemorrhage. Conclusion: magnetic sensitive weighted images can detect cerebral microhaemorrhage and reflect the pathological changes of microvascular lesions. Microhemorrhage occurred in cortex and basal ganglia where R2STAR value increased, and the location of hemorrhage was basically consistent with pathology. Using ESWAN scan, the change of R2STAR value may predict spontaneous intracerebral hemorrhage in hypertension.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R743.34;R445.2
[Abstract]:Objective: to study the relationship between magnetic sensitivity weighted image and pathology in detecting cerebral microhemorrhage. To investigate the correlation between R2STAR value of ESWAN and cerebral microhemorrhage. To determine whether the sudden increase of R2STAR in cortical and basal ganglia on MRI-ESWAN scan can predict cerebral microhemorrhage 1-2 weeks ahead of time. Materials and methods: thirty aged male (SHR) rats with stroke susceptibility to spontaneous hypertension were obtained and their blood pressure was continuously monitored and MRI scans were performed regularly according to the experimental plan. Systolic blood pressure (SBP) and diastolic blood pressure (DBP).) were recorded in each SHR rat using a blood pressure monitor Using animal coil scanning and MRI scanning sequences including ESWAN,3D-FRSG and T2-WI, the R2STAR values in ESWAN were measured, and the phase map and / or microbleeds were observed in the brain. The time and location of microhemorrhage were recorded. ESWAN sequence phase map and / or amplitude map showed that microhemorrhage or haemorrhage was positive in any part of the brain, but no microhemorrhage or haemorrhage was found in negative group. The rats were killed after ESWAN found intracerebral hemorrhage. The pathological sections of brain tissue were observed and compared with MRI images. No bleeding group was killed at the end of the experiment. Repeated measurement variance analysis was used to compare the R2STAR values of different parts at different time before bleeding. Results: there were 17 cases of spontaneous cerebral hemorrhage, 9 cases of no hemorrhage and 4 cases of death in the male model of stroke susceptible spontaneous hypertension (SHR). There was no significant difference in blood pressure between positive group and negative group (P0.05). The R2STAR values of cortex and basal ganglia in the positive group were higher than those in the negative group (P0.05). The R2STAR values of the lesion side and the contralateral area in the 38-68W positive group were increased steadily. In the 68-74W positive group, the R2STAR value in the central region of the lesion increased rapidly (P0.05), and the change of the contralateral mirror phase was relatively gentle. In the positive group, the R2STAR values of cerebral cortex and basal ganglia were significantly higher than those of the contralateral area before hemorrhage (P0.05). The distribution of microhaemorrhage or haemorrhage in post-processing ESWAN images was basically the same as that observed by pathological sections, and the location where the R2STAR value of ESWAN increased was basically the same as that of subsequent intracerebral microhaemorrhage. Conclusion: magnetic sensitive weighted images can detect cerebral microhaemorrhage and reflect the pathological changes of microvascular lesions. Microhemorrhage occurred in cortex and basal ganglia where R2STAR value increased, and the location of hemorrhage was basically consistent with pathology. Using ESWAN scan, the change of R2STAR value may predict spontaneous intracerebral hemorrhage in hypertension.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R743.34;R445.2
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