小鼠脑血流量自动调节范围观察

发布时间：2018-12-15 06:57

【摘要】：目的明确小鼠脑血流自动调节的范围。方法以左颈动脉插管测定的动脉血压为标准,建立小鼠股动脉插管监测动脉血压的动物模型。在脑血流量测定组模型中通过放血及尾静脉泵入去甲肾上腺素的方式逐步分级升高和降低其动脉血压,以10~15 mm Hg为一个梯度,同时采用激光多普勒监测模型大脑中动脉区域脑血流量。结果小鼠股动脉插管与左颈动脉插管监测的收缩压、舒张压和平均动脉相近(P均0.05),但脉压差异较大(P0.05)。脑血流量测定组模型平均动脉压在-30~+30 mm Hg(即平均动脉压降低或升高30 mm Hg)变化时,其脑血流量无明显改变(P均0.05);超过该范围后,脑血流量随动脉血压升高而升高(P均0.05),随动脉血压下降而下降(P均0.05)。结论小鼠脑血流自动调节范围为平均动脉压-30~+30 mm Hg,超出此范围后,脑血流量随着血压的改变而明显改变。
[Abstract]:Objective to determine the range of automatic cerebral blood flow regulation in mice. Methods based on the arterial blood pressure measured by left carotid artery catheterization, an animal model of monitoring arterial blood pressure by femoral artery catheterization in mice was established. In the cerebral blood flow measurement group, the arterial blood pressure was gradually increased and decreased by bleeding and injecting norepinephrine into the caudal vein, with a gradient of 10 ~ 15 mm Hg. At the same time, the regional cerebral blood flow of middle cerebral artery was monitored by laser Doppler. Results the systolic blood pressure, diastolic pressure and mean arterial pressure were similar between femoral artery intubation and left carotid artery intubation in mice (P 0.05), but the pulse pressure was significantly different (P0.05). In the cerebral blood flow measurement group, the mean arterial pressure decreased or increased for 30 mm Hg) after -30 mm Hg (, but there was no significant change in the cerebral blood flow (all P 0. 05). Beyond this range, the cerebral blood flow increased with the increase of arterial blood pressure (P 0.05) and decreased with the decrease of arterial blood pressure (P 0.05). Conclusion the range of automatic cerebral blood flow regulation in mice is 30 ~ 30 mm Hg, after the mean arterial pressure exceeds this range, and the cerebral blood flow changes obviously with the change of blood pressure.
【作者单位】：广州医科大学附属第二医院;
【基金】：国家自然科学基金资助项目(831371573)
【分类号】：R743

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