BAG3蛋白在人脑原、复发胶质瘤中的表达情况及相关性研究

发布时间：2018-12-16 13:59

【摘要】：第一部分BAG3蛋白在原发性人脑胶质瘤表达情况目的：研究正常脑组织、不同级别人脑原发性胶质瘤组织中BAG3蛋白表达情况，从而探讨其在人脑胶质瘤细胞存活、增殖、侵袭性中的可能作用。方法：应用免疫组织化学方法分别检测正常脑组织5例，人脑胶质瘤I级4例，II级17例，III级19例、IV级18例，并于显微镜下观察BAG3蛋白表达及分布情况。应用Western-blot检测II、III、IV级别胶质瘤BAG3蛋白表达量。结果：正常脑组织中BAG3蛋白不表达，不同级别胶质瘤中，均可以看到有BAG3蛋白的表达，BAG3蛋白分布在细胞浆中，细胞核中不表达。在某些高级别胶质瘤组织中，观察到BAG3蛋白阳性细胞分布不均匀，并观察到一些血管内皮细胞表达BAG3蛋白。各级别胶质瘤中，，均有BAG3蛋白低表达与高表达情况。BAG3蛋白表达，在III、IV级胶质瘤较I~II级胶质瘤显著增高（P0.05），在III、IV级胶质瘤之间无显著差异（P0.05）。BAG3蛋白表达水平与病理级别呈正相关（r=0.419,P0.01）。结论：BAG3蛋白在人脑正常组织中不表达，在胶质瘤组织中的表达，并且在高级别胶质瘤中表达较低级别胶质瘤中明显升高，表达水平与病理分级呈正相关。BAG3蛋白在人脑胶质瘤中的表达，可能与胶质瘤细胞存活、抗凋亡、增殖、侵袭性有关。第二部分BAG3蛋白在原、复发人脑胶质瘤中的表达情况目的：研究同一胶质瘤病人原发性胶质瘤与复发性胶质瘤组织中BAG3蛋白表达情况，比较原发与复发胶质瘤BAG3蛋白表达有无变化，从而阐明BAG3蛋白表达是否与胶质瘤原、复发相关。方法：用免疫组织化学方法检测12例胶质瘤病人原、复发胶质瘤组织中BAG3蛋白表达情况，并于显微镜下观察染色结果。结果：在原、复发胶质瘤组织中，BAG3蛋白表达无显著差异（P0.05）。结论： BAG3蛋白表达与人脑胶质瘤原、复发之间没有相关。全篇结论：BAG3蛋白在正常脑组织中不表达，在人脑胶质瘤中表达，在高级别中表达较低级别明显增高。BAG3蛋白表达情况与病理分级呈正相关。BAG3蛋白表达与胶质瘤原、复发之间没有明显相关。胶质瘤血管内皮细胞表达BAG3蛋白，可能与肿瘤血管生长、维持其生存，以及与胶质瘤侵袭性有关。
[Abstract]:Part one: expression of BAG3 protein in human glioma objective: to study the expression of BAG3 protein in normal brain tissue and human glioma tissues of different grades, and to investigate the survival and proliferation of BAG3 protein in human glioma cells. The possible role of invasiveness. Methods: immunohistochemical method was used to detect the expression and distribution of BAG3 protein in 5 cases of normal brain tissue, 4 cases of human glioma grade I, 17 cases of II grade, 19 cases of III grade and 18 cases of IV grade. The expression of BAG3 protein in II,III,IV grade gliomas was detected by Western-blot. Results: there was no expression of BAG3 protein in normal brain tissue, but no expression of BAG3 protein was observed in gliomas of different grades. BAG3 protein was distributed in cytoplasm and not expressed in nucleus. Uneven distribution of BAG3 protein positive cells and expression of BAG3 protein in some vascular endothelial cells were observed in some high grade glioma tissues. The expression of BAG3 protein was significantly higher in III,IV grade gliomas than in I~II grade gliomas (P0.05), and in III, the expression of BAG3 protein was higher than that of I~II grade gliomas (P0.05). There was no significant difference between IV grade gliomas (P0.05). The expression of BAG3 protein was positively correlated with pathological grade (r = 0.419, P0.01). Conclusion: BAG3 protein was not expressed in normal human brain tissues, but increased in glioma tissues, and increased significantly in high grade gliomas and lower grade gliomas. The expression of BAG3 protein in human glioma may be related to the survival, anti-apoptosis, proliferation and invasiveness of glioma cells. The second part of the expression of BAG3 protein in proto-recurrent human glioma objective: to study the expression of BAG3 protein in primary glioma and recurrent glioma tissues of the same glioma patients. The expression of BAG3 protein in primary and recurrent gliomas was compared to clarify whether the expression of BAG3 protein was related to the proto and recurrence of gliomas. Methods: immunohistochemical method was used to detect the expression of BAG3 protein in 12 patients with glioma, and the staining results were observed under microscope. Results: there was no significant difference in the expression of BAG3 protein in primary and recurrent gliomas (P 0.05). Conclusion: there is no correlation between the expression of BAG3 protein and the proto-recurrence of human glioma. Conclusion: BAG3 protein is not expressed in normal brain tissue, but it is expressed in human glioma. The expression of BAG3 protein is positively correlated with the pathological grade. The expression of BAG3 protein is positively correlated with the proto-glioma. There was no significant correlation between recurrence. The expression of BAG3 protein in glioma vascular endothelial cells may be related to tumor angiogenesis, survival and invasiveness of glioma.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.41

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