瘦素对局灶性脑缺血后内质网应激相关蛋白的作用

发布时间：2018-12-31 07:47

【摘要】：目的探讨瘦素对大鼠局灶性脑缺血后内质网应激相关蛋白的影响。方法 SD大鼠40只,随机分为假手术组、脑缺血组、脑缺血瘦素预处理组,线栓法制备大鼠大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,在闭塞血管前3 h皮下注射瘦素,于闭塞血管6 h后采用Longa 5分制量表进行神经功能评分、称量体重及脑水肿变化,并灌注取脑,采用免疫荧光化学法检测内质网应激相关蛋白葡萄糖调节蛋白78(glucoseregulated protein 78,GRP78)及C/EBP同源蛋白(C/EBP-homologous protein,CHOP)表达。结果脑缺血瘦素预处理组和脑缺血组体重变化未见差异(P0.05);脑缺血瘦素预处理组神经功能评分(1.90±0.31 vs.2.50±0.52,P0.05)和脑水肿程度(3.60±0.52 vs.7.70±0.94,P0.001)明显轻于脑缺血组,同时脑缺血瘦素预处理组GRP78表达明显高于脑缺血组(48.69±5.06 vs.35.78±4.35,P0.01),CHOP表达明显低于脑缺血组(38.81±5.34 vs.60.24±4.11,P0.01)。结论瘦素可减少神经功能缺损并可能与其上调GRP78蛋白,下调CHOP蛋白来减弱脑缺血导致的内质网应激有关。
[Abstract]:Objective to investigate the effect of leptin on endoplasmic reticulum stress-related protein after focal cerebral ischemia in rats. Methods Forty SD rats were randomly divided into sham-operated group and ischemic leptin preconditioning group. The rat model of middle cerebral artery occlusion (middle cerebral artery occlusion,MCAO) was established by thread occlusion. Leptin was injected subcutaneously 3 hours before occlusion. After 6 hours of occlusion, nerve function score, weight and brain edema were measured by Longa 5 score scale, and brain was taken by perfusion. The endoplasmic reticulum stress-related protein glucose-regulated protein 78 (glucoseregulated protein 78 was detected by immunofluorescence method. GRP78 and C/EBP homologous protein (C/EBP-homologous protein,CHOP). Results there was no significant difference in body weight between cerebral ischemic preconditioning group and cerebral ischemia group (P0.05). The neurological function score (1.90 卤0.31 vs.2.50 卤0.52P 0.05) and the degree of cerebral edema (3.60 卤0.52 vs.7.70 卤0.94g P0.001) in the leptin preconditioning group were significantly lower than those in the ischemic group. Meanwhile, the expression of GRP78 in ischemic preconditioning group was significantly higher than that in ischemic group (48.69 卤5.06 vs.35.78 卤4.35 vs.35.78 P0.01), and the expression of), CHOP in ischemic group was significantly lower than that in ischemic group (38.81 卤5.34 vs.60.24 卤4.11P0.01). Conclusion leptin can reduce neurologic impairment and may be related to the decrease of endoplasmic reticulum stress induced by cerebral ischemia by up-regulating GRP78 protein and down-regulating CHOP protein.
【作者单位】：广西医科大学第一附属医院神经内科;
【基金】：广西壮族自治区教育厅科研课题(编号:YB2014069) 广西壮族自治区医疗卫生适宜技术开发与推广应用课题(编号:S201656)
【分类号】：R743.3

【参考文献】