钾通道Kv4.2、Kv1.4在硫化氢后处理对大鼠短暂全脑缺血神经保护中的作用研究

发布时间：2019-01-05 08:10

【摘要】：目的研究硫氢化钠(sodium hydrosulfide,Na HS)后处理对短暂全脑缺血大鼠海马中钾通道Kv4.2和Kv1.4 mRNA表达变化的影响及其脑保护作用,从而探讨Na HS对大鼠短暂全脑缺血神经保护作用的机制。方法用4VO方法建立大鼠短暂性全脑缺血(transient global cerebral ischemia,t GCI)模型,大鼠被随机分配到3组,分别为:假手术组(sham)、t GCI组、Na HS后处理组。Na HS后处理组为t GCI之后1 d,给予大鼠腹腔注射Na HS 24μmmol/kg或者180μmmol/kg。通过尼氏染色与Neu N免疫染色确定海马神经元的死亡,通过RT-PCR方法检测海马组织Kv4.2和Kv1.4mRNA水平的表达变化。结果 (1)与t GCI组比较,在t GCI之后1 d给予24μmol/kg Na HS后处理使海马CA1区存活细胞数目显著增加,而高剂量的Na HS(180μmol/kg)后处理对t GCI大鼠海马CA1区则无明显的保护作用。(2)在Re 26 h和Re 48 h,海马组织中Kv4.2、Kv1.4的mRNA表达水平均明显低于假手术组(P0.05)。在Re 26 h+Na HS组,kv4.2(1.24±0.08)和kv1.4(1.11±0.07)的mRNA表达水平均分别高于Re 26 h组的kv4.2(0.75±0.04)和kv1.4(0.79±0.06),差异均有显著性(P0.05)。结论外源性Na HS可能通过上调大鼠t GCI后海马区Kv4.2和Kv1.4 mRNA的表达,从而导致膜电位超极化,降低神经元兴奋性和氧耗,继而保护神经元免受脑缺血损伤。
[Abstract]:Objective to study the effects of post-treatment with sodium sulfide (sodium hydrosulfide,Na HS) on the expression of potassium channel Kv4.2 and Kv1.4 mRNA in hippocampus of rats with transient global cerebral ischemia. To explore the mechanism of neuroprotective effect of Na HS on transient global cerebral ischemia in rats. Methods the model of transient global cerebral ischemia (transient global cerebral ischemia,t GCI) was established by 4VO in rats. The rats were randomly assigned to three groups: sham operation group, (sham), t GCI group, Na HS post-treatment group,. Na HS post-treatment group, 1 day after t GCI. Rats were given intraperitoneal injection of Na HS 24 渭 mmol/kg or 180 渭 mmol/kg. The death of hippocampal neurons was determined by Nissl staining and Neu N immunostaining. The expression of Kv4.2 and Kv1.4mRNA in hippocampal tissue was detected by RT-PCR method. Results (1) compared with t GCI group, 24 渭 mol/kg Na HS post-treatment on day 1 after t GCI significantly increased the number of surviving cells in CA1 area of hippocampus. However, high dose of Na HS (180 渭 mol/kg) had no obvious protective effect on CA1 area of hippocampus of t GCI rats. (2) Kv4.2, in hippocampus of Re 26 h and Re 48 h. The expression of mRNA in Kv1.4 was significantly lower than that in sham operation group (P0.05). In Re 26 h Na HS group, the mRNA expression levels of kv4.2 (1.24 卤0.08) and kv1.4 (1.11 卤0.07) were higher than those of Re 26 h kv4.2 (0.75 卤0.04) and kv1.4 (0.79 卤0.06), respectively. The difference was significant (P0.05). Conclusion exogenous Na HS may up-regulate the expression of Kv4.2 and Kv1.4 mRNA in hippocampus after t GCI in rats, which may lead to the hyperpolarization of membrane potential, decrease the excitability and oxygen consumption of neurons, and then protect neurons from cerebral ischemia.
【作者单位】：青海大学附属医院神经内科;青海大学研究生院;
【基金】：青海省科技厅应用基础研究计划(No.2016-ZJ-735)
【分类号】：R743.31

【参考文献】