基于NIRS-ICG的脑血流量无创测量

发布时间：2019-01-05 11:22

【摘要】：临床上脑血流量(cerebral blood flow,CBF)等脑血管血流动力学参数是脑血氧水平及脑血管储备功能诊断依据,现有检测手段存在技术复杂及相应试剂或设备不适用于所有诊断人群等缺点。为解决以上问题,利用近红外光谱技术(NIRS)结合吲哚青绿(indocyanine green,ICG)脉搏色素浓度法,研究了一种无创、快速、可重复测量的脑血流量床旁检测方法 NIRS-ICG。该方法根据静脉注射ICG后脑组织及脑动脉血流中三种主要吸光色团氧合血红蛋白(oxygenated hemoglobin,HbO_2)、还原血红蛋白(reduced hemoglobin,HbR)及ICG的浓度变化情况,建立脑组织及脑动脉血流中ICG积累量及引入量模型,以获得脑血氧及CBF等脑血流动力学参数。为验证该方法的可行性,将NIRS-ICG应用于血碳酸正常及高碳酸血症病理模型的实验猪的脑血流情况检测。具体方法是:分别对四组实验猪用按0%,3%,6%,9%比例调制的CO_2和空气混合气体施行机械通气,静脉快速推注ICG后,利用NIRS-ICG方法测量CBF、脑动脉血氧饱和度(cerebral arterial oxygen saturation,SaO_2)及脑血管管床平均循环时间(mean transit time,MTT)。实验结果表明,NIRS-ICG测得的CBF随CO_2比率升高而升高,SaO_2随着CO_2比例的升高而降低,MTT并无显著变化,与生理变化一致。因此,该方法可为脑血氧及脑血管储备功能诊断提供可靠依据。
[Abstract]:Cerebral vascular hemodynamic parameters such as cerebral blood flow (cerebral blood flow,CBF) are the diagnostic basis of cerebral blood oxygen level and cerebrovascular reserve function. The existing detection methods have some shortcomings, such as technical complexity and the corresponding reagent or equipment can not be applied to all diagnostic population and so on. In order to solve the above problems, a noninvasive, fast and repeatable method for the bedside measurement of cerebral blood flow (NIRS-ICG.) was studied by using near infrared spectroscopy (NIRS) combined with indocyanine green (indocyanine green,ICG) pulse pigment concentration method. This method was based on the changes of the concentrations of three main absorbent groups of oxygenated hemoglobin (oxygenated hemoglobin,HbO_2), reductive hemoglobin (reduced hemoglobin,HbR) and ICG in cerebral tissue and cerebral artery blood flow after intravenous injection of ICG. In order to obtain cerebral hemodynamic parameters such as cerebral blood oxygen and CBF, a model of ICG accumulation and introduction in cerebral tissue and cerebral artery blood flow was established. In order to verify the feasibility of this method, NIRS-ICG was applied to the detection of cerebral blood flow in pigs with normal blood carbonate and hypercapnia pathological model. The specific methods were as follows: the four groups of pigs were mechanically ventilated with CO_2 and air mixed gases, which were modulated by 0% and 6%, respectively. After ICG was injected rapidly through vein, CBF, was measured by NIRS-ICG method. Cerebral arterial oxygen saturation (cerebral arterial oxygen saturation,SaO_2) and cerebrovascular bed mean circulation time (mean transit time,MTT). The results showed that the CBF measured by NIRS-ICG increased with the increase of CO_2 ratio, SaO_2 decreased with the increase of CO_2 ratio, and MTT did not change significantly, which was consistent with physiological changes. Therefore, this method can provide reliable basis for the diagnosis of cerebral blood oxygen and cerebrovascular reserve function.
【作者单位】：吉林大学仪器科学与电气工程学院;吉林大学口腔医学院;
【基金】：国家“十二五”科技支撑计划(2015BAI02B04) 国家自然科学基金项目(21207047) 吉林省发改委省级产业创新专项资金项目(2016C052-2) 吉林省教育厅“十二五”科学技术研究规划重点项目(440020031134)资助
【分类号】：R741.04

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