丰富环境对脑缺血大鼠行为学恢复及神经丝蛋白、突触素表达的影响

发布时间：2019-01-09 11:06

【摘要】：目的:研究"形神共养"对脑缺血大鼠行为学恢复及神经丝蛋白(NF)、突触素(SYN)表达的影响。方法:制作线栓法大鼠脑缺血模型(MCAO),随机分为"形养"组、"神养"组、"形神共养"组、"标准养"组及假手术组。术后第3、10、17、24、30天进行平衡木行走试验及倾斜板试验,干预结束后进行免疫组织化学检测大鼠梗死周围皮质及海马区NF、SYN的表达。结果:平衡木行走试验和倾斜板试验:三个治疗组大鼠评分均优于"标准养"组(P0.05)。免疫组化检测:"形养"、"神养"、"形神共养"组大鼠梗死周围皮质及海马区NF、SYN表达均高于标准养组,"形神共养"组表达高于其他两组(P0.05)。结论:"形神共养"的丰富生存环境可促进脑缺血大鼠行为学恢复,其机制可能与促进NF及SYN的表达有关。
[Abstract]:Aim: to study the effects of "form and mind co-nourishment" on behavioral recovery and expression of neurofilament protein (NF), synaptophysin (SYN) in rats with cerebral ischemia. Methods: (MCAO), was randomly divided into three groups: "form nourishing" group, "spirit nourishing" group, "form and spirit co-nourishing" group, "standard nourishing" group and sham operation group. The balance beam walking test and tilt plate test were carried out on the 30th day after operation. After the intervention, the expression of NF,SYN in the cortex and hippocampus around the infarction was detected by immunohistochemistry. Results: balance beam walking test and tilting plate test: the scores of the three treatment groups were better than those of the "standard nourishing" group (P0.05). Immunohistochemical examination showed that the expression of NF,SYN in cortex and hippocampus of rats in the group of "form nourishing", "spirit nourishing" and "form and spirit co-nourishing" was higher than that of the standard group, and the expression of NF,SYN in the group of "form and spirit co-nourishing" was higher than that of the other two groups (P0.05). Conclusion: the rich living environment of "form and spirit co-nourishing" can promote the recovery of behavior in rats with cerebral ischemia, and its mechanism may be related to promoting the expression of NF and SYN.
【作者单位】：武汉大学中南医院康复医学科武汉大学脑血管病研究中心;武汉大学中南医院康复医学科;
【基金】：国家自然科学基金资助项目(303163421)
【分类号】：R743.3

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