运动训练对脑梗死大鼠梗死边缘区神经细胞自噬及凋亡的影响

发布时间：2019-01-12 09:21

【摘要】：目的:研究运动训练对脑梗死急性期大鼠神经功能恢复及梗死边缘区皮质神经细胞自噬及凋亡的影响。方法:建立大脑中动脉闭塞再灌注模型,并分成运动训练组、对照组和假手术组。运动训练组大鼠给予运动训练;对照组与假手术组则不予以任何针对性训练。造模术后第3、7、14d采用改良神经损伤程度评分(mNSS)对各组大鼠进行功能评估,同时以尼氏染色法测量脑梗死体积,免疫荧光方法观察梗死边缘区皮质自噬标志物LC3-Ⅱ及凋亡标志物TUNEL的表达情况。结果:造模术后14d运动训练组大鼠mNSS及脑梗死体积均优于对照组,差别具有显著性意义(P0.05)。运动训练组大鼠梗死边缘区皮质LC3-Ⅱ阳性细胞数在造模后14d少于对照组(P0.05)。相关性分析结果显示LC3-Ⅱ阳性细胞数与mNSS(r=0.901,P0.001)及梗死体积(r=0.832,P0.001)成正相关。TUNEL阳性细胞数在造模后7、14d均少于对照组(P0.001),且44.6%的LC3-Ⅱ与TUNEL存在共定位。结论:运动训练能够促进脑梗死急性期大鼠神经功能恢复,其机制可能与其减少梗死边缘区神经细胞自噬及凋亡有关。
[Abstract]:Aim: to study the effects of exercise training on the recovery of neural function and the autophagy and apoptosis of cortical neurons in the acute phase of cerebral infarction in rats. Methods: middle cerebral artery occlusion reperfusion model was established and divided into exercise training group, control group and sham operation group. Rats in the exercise training group were given exercise training, while the control group and the sham operation group were not given any specific training. The function of rats in each group was evaluated by modified nerve injury score (mNSS) on the 3rd day and 14th day after operation, and the infarct volume was measured by Nissl's staining method. The expression of autophagy marker LC3- 鈪，

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