LOC389023在难治性颞叶癫痫中分子海绵机制的预测

发布时间：2019-02-25 21:34

【摘要】：【目的】研究可能与LOC389023和二肽基肽酶10(DPP10)作用的micro RNA在难治性颞叶癫痫患者皮质中的表达变化,预测LOC389023在颞叶癫痫中的分子海绵机制。【方法】15例脑外伤患者切除的皮质为正常组,26例难治性颞叶癫痫患者行癫痫灶切除的皮质为癫痫组。运用western blot、免疫组织化学染色检测DPP10和电压依赖性钾通道4.3(Kv4.3)的表达变化,采用免疫荧光染色对DPP10和kv4.3进行定位,采用免疫共沉淀分析DPP10与Kv4.3的相关性。运用miRanda、Pita、Target Scan和miRDB软件分别预测与LOC389023以及DPP10相互作用的micro RNA。采用q PCR技术检测LOC389023以及micro RNA的表达变化。【结果】免疫组织化学染色和western blot分析结果显示,DPP10在癫痫组中高表达(P0.05),Kv4.3则低表达(P0.05);DPP10与Kv4.3共表达于神经元的细胞膜且两者有相互作用;通过软件预测得到5个候选micro RNA(miR-32-5p,miR-140-5p,miR-367-3p,miR-25-3p和miR-4325);q PCR检测结果显示:在癫痫组中,LOC389023和miR-140-5p表达水平均低于对照组(P0.05),miR-25-3p和miR-367-3p表达水平均高于对照组(P0.05),而miR-32-5p和miR-4325无明显变化(P0.05)。【结论】LOC389023可能以分子海绵的机制调控miR-25-3p和miR-367-3p,继而调控DPP10的表达参与癫痫的形成。
[Abstract]:[objective] to study the expression of LOC389023 and dipeptidyl peptidase 10 (DPP10) in the cortex of patients with refractory temporal lobe epilepsy. The molecular sponge mechanism of LOC389023 in temporal lobe epilepsy was predicted. [methods] Fifteen patients with brain trauma were treated with normal cortex, and 26 patients with refractory temporal lobe epilepsy were treated with epilepsy. The expression of DPP10 and voltage-dependent potassium channel 4.3 (Kv4.3) was detected by western blot, immunohistochemical staining, DPP10 and kv4.3 were localized by immunofluorescence staining, and the correlation between DPP10 and Kv4.3 was analyzed by immunoprecipitation. Prediction of micro RNA. interacting with LOC389023 and DPP10 by miRanda,Pita,Target Scan and miRDB Q-PCR technique was used to detect the expression of LOC389023 and micro RNA. [results] Immunohistochemical staining and western blot analysis showed that the expression of DPP10 was high in epilepsy group (P0.05), while Kv4.3 was lower (P0.05). DPP10 and Kv4.3 co-expressed in the membrane of neurons and interacted with each other, and five candidate micro RNA (miR-32-5p,miR-140-5p,miR-367-3p,miR-25-3p and miR-4325 were predicted by software. The results of Q-PCR showed that the expression levels of LOC389023 and miR-140-5p in epilepsy group were lower than those in control group (P0.05), while the expression levels of miR-25-3p and miR-367-3p in epilepsy group were higher than those in control group (P0.05). [conclusion] LOC389023 may regulate the expression of miR-25-3p and miR-367-3p, by molecular sponge, and then regulate the expression of DPP10, which may be involved in the formation of epilepsy. [conclusion] miR-32-5p and miR-4325 do not change significantly (P0.05).
【作者单位】：重庆医科大学附属第一医院神经外科;山东大学齐鲁医院神经内科;
【基金】：重庆市卫生局重点资助项目(2013-1-005)
【分类号】：R742.1

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