雷帕霉素抗神经胶质瘤的活性及其机制

发布时间：2019-03-12 14:03

【摘要】：目的探讨雷帕霉素抗神经胶质瘤的肿瘤活性及其相关作用机制。方法 MTT细胞毒实验检测雷帕霉素对神经胶质瘤细胞C6的生长抑制活性,Annexin V/PI双染流式细胞仪检测细胞凋亡的发生,PI单染流式细胞仪检测细胞周期的变化,提取细胞总RNA,RT-PCR检测survivin mRNA的表达变化,提取细胞总蛋白Western印迹检测survivin蛋白的表达变化。结果雷帕霉素对神经胶质瘤细胞C6显示了高效的抗肿瘤活性,IC50值为(8.34±0.62)μmol/L。流式细胞仪凋亡分析显示0、5、10、20μmol/L雷帕霉素作用C6细胞48 h后,细胞的凋亡率分别为(3.56%±1.04%)、(13.34%±3.86%)、(22.84%±4.53%)、(51.91%±6.29%),各组间有统计学差异(P0.01)。细胞周期分析显示0、5、10、20μmol/L雷帕霉素处理C6细胞48h后,细胞增殖指数分别为(64.54%±6.04%)、(50.24%±4.86%)、(39.26%±5.61%)、(30.08%±6.42%)。RT-PCR和Western印迹检测显示雷帕霉素可以下调survivin mRNA和蛋白的表达。结论雷帕霉素具有高效的抗神经胶质瘤活性,其作用机制可能与其抑制了m TOR信号通路下调survivin靶蛋白的表达有关。
[Abstract]:Objective to investigate the anti-glioma activity of rapamycin and its mechanism. Methods MTT cytotoxicity assay was used to detect the inhibitory activity of rapamycin on C6 glioma cells, Annexin V/PI double staining flow cytometry was used to detect apoptosis, and PI single staining flow cytometry was used to detect cell cycle changes. Total RNA, was extracted from glioma cells. The expression of survivin mRNA was detected by RT-PCR, and the expression of survivin was detected by Western blot. Results rapamycin showed high antitumor activity against C6 glioma cells, IC _ (50) was (8.34 卤0.62) 渭 mol/L.. The apoptosis rate of C6 cells treated with 0, 5, 10, 20 渭 mol / L rapamycin for 48 h was 3.56% 卤1.04%), (13.34% 卤3.86%), (22.84% 卤4.53%, respectively, and the apoptosis rate of C6 cells treated with rapamycin was 3.56% 卤1.04%, 13.34% 卤3.86%, 22.84% 卤4.53%, respectively. (51.91% 卤6.29%), there was statistical difference among the groups (P0.01). Cell cycle analysis showed that the proliferation index of C6 cells treated with 0, 5, 10 and 20 渭 mol / L rapamycin for 48 h was 64.54% 卤6.04%), (50.24% 卤4.86% 卤4.86%), (39.26% 卤5.61%, respectively. (30.08% 卤6.42%). RT-PCR and Western blot analysis showed that rapamycin could down-regulate the expression of survivin mRNA and protein. Conclusion rapamycin has high anti-glioma activity and its mechanism may be related to its inhibition of m-TOR signaling pathway down-regulating the expression of survivin target protein.
【作者单位】：青海省第五人民医院;
【分类号】：R739.41

【相似文献】