缺血性脑卒中患者外周血miRNA生物信息学分析研究

发布时间：2019-04-02 11:03

【摘要】：目的应用生物信息学技术分析缺血性脑卒中患者外周血中微小RNA(miRNA)的靶基因及其功能、在疾病中的作用。方法在GEO数据库中检索缺血性脑卒中基因芯片数据,并利用生物信息学工具筛选差异表达的miRNA,对差异表达的miRNA进行靶基因预测分析,对miRNA靶基因进行生物学功能分析及信号通路分析,最后构建miRNA调控网络和miRNA靶基因调控网络。结果检索到芯片数据GSE55937,筛选得到50个差异表达的miRNA。筛选出的miRNA的靶基因的合集共7 557个。这些靶基因的功能主要为DNA依赖转录、DNA依赖转录调节等,主要分布在丝裂原活化蛋白激酶信号通路、肿瘤信号通路等信号通路。miRNA功能的调控分析及miRNA靶基因调控网络图提示,人类miRNA(hsa-miR)-15a-5p、hsa-miR-106b-5p、hsa-let-7c-5p、hsa-let-7i-5p、hsa-let-7g-5p中心度最高。结论缺血性脑卒中患者外周血中存在差异性表达的miRNA,hsa-miR-15a-5p、hsa-miR-106b-5p、hsa-let-7c-5p、hsa-let-7i-5p、hsa-let-7g-5p与缺血性脑卒中的发病密切相关。
[Abstract]:Objective to analyze the target gene and its function of micro-RNA (miRNA) in peripheral blood of patients with ischemic stroke by bioinformatics technique, and to explore the role of microgene in the disease. Methods the gene chip data of ischemic stroke were retrieved in GEO database, and the differentially expressed miRNA, was screened by bioinformatics tools to predict and analyze the target genes of the differentially expressed miRNA. The biological function and signal pathway of miRNA target gene were analyzed. Finally, miRNA regulatory network and miRNA target gene regulatory network were constructed. Results 50 differentially expressed miRNA. were obtained by GSE55937, screening of microarray data. A total of 7 557 miRNA target genes were screened. The functions of these target genes are mainly DNA-dependent transcription, DNA-dependent transcriptional regulation, and mainly distributed in mitogen-activated protein kinase signaling pathway. Analysis of the regulation of miRNA function and miRNA target gene regulatory network suggested that human miRNA (hsa-miR)-15a-5p, hsa-miR-106b-5p, hsa-let7c-5p, hsa-let-7i-5p, hsa-let-15a-5p, hsa-mir-106b-5p, hsa-let-7i-5p, The centrality of hsa-let-7g-5p was the highest. Conclusion there is a differential expression of miRNA,hsa-miR-15a-5p,hsa-miR-106b-5p,hsa-let-7c-5p,hsa-let-7i-5p, in peripheral blood of patients with ischemic stroke. Hsa-let-7g-5p is closely related to ischemic stroke.
【作者单位】：广西医科大学附属民族医院神经内二科;广西医科大学第一附属医院神经内科;
【基金】：国家自然科学基金(81360191) 广西医药卫生科研课题(Z2015105)
【分类号】：R743.3

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