不同疗程高压氧治疗对脑出血大鼠血肿周围AQP4和SOD表达的影响

发布时间：2019-06-10 20:43

【摘要】：目的:探究不同疗程高压氧(HBO)治疗对脑出血(ICH)大鼠血肿周围水通道蛋白(AQP4)及超氧化物歧化酶(SOD)表达的影响。方法:将52只雄性SD大鼠随机分为3组,正常组(12只)、脑出血组(20只)和高压氧治疗组(20只),脑出血模型采用胶原酶诱导法制备。各组大鼠根据不同高压氧治疗疗程再分为4个亚组:1周组、2周组、3周组和4周组,正常组每组3只大鼠,脑出血组和高压氧治疗组每组5只大鼠。高压氧治疗组于脑出血术后6h对模型制备成功的大鼠给予压力为2.0ATA,稳压吸氧时间60min的HBO治疗,1次/d,每周7次。正常组和脑出血组不予任何干预措施,所有大鼠饲养在正常环境中,各组大鼠分别在对应治疗周期结束后采用断头取脑法保留血肿周围脑组织留取标本。采用干湿比重法测定脑组织的水含量,免疫组化法测定AQP4的表达,黄嘌呤氧化酶法测定SOD的活力。结果:(1)脑组织的水含量:脑出血组及高压氧治疗组脑组织的水含量在1—3周均高于正常组(P0.05);高压氧治疗组较脑出血组脑组织的水含量均减少(P0.05);高压氧治疗组间1周、3周、4周脑组织水含量均低于2周(P0.05),该三组之间差异不明显(P0.05)。(2)脑组织AQP4的表达:脑出血组及高压氧治疗组脑组织AQP4表达在1—3周均高于正常组(P0.05);高压氧治疗组较脑出血组1—3周脑组织AQP4表达均减少(P0.05);高压氧治疗组间第1周及第3周与第2周相比表达较低(P0.05)。(3)脑组织SOD的表达:脑出血组及高压氧治疗组SOD表达在1—3周均高于正常组(P0.05),高压氧治疗组较脑出血组相比SOD表达均有增加(P0.05);高压氧治疗组间SOD表达1周、2周、3周均高于4周(P0.05),该3组之间相比无差异(P0.05)。结论:高压氧治疗可下调AQP4表达减少血肿周围脑组织水含量,上调SOD表达,且连续高压氧治疗4周优于其他疗程。
[Abstract]:Aim: to investigate the effects of different courses of hyperbaric oxygen (HBO) on the expression of aquaporin (AQP4) and SOD (SOD) around hematomas in rats with intracerebral hemorrhage (ICH). Methods: 52 male SD rats were randomly divided into three groups: normal group (n = 12), cerebral hemorrhage group (n = 20) and hyperbaric oxygen treatment group (n = 20). The model of cerebral hemorrhage was induced by collagenase. According to different courses of hyperbaric oxygen therapy, rats in each group were divided into four subgroups: 1 week group, 2 week group, 3 week group and 4 week group, 3 rats in each group, 5 rats in cerebral hemorrhage group and 5 rats in hyperbaric oxygen treatment group. The rats in the hyperbaric oxygen treatment group were treated with HBO with pressure 2.0 ATA and 60min once a day 7 times a week at 6 h after intracerebral hemorrhage (ICH). There were no intervention measures in the normal group and the cerebral hemorrhage group. All the rats were kept in the normal environment. After the corresponding treatment cycle, the brain tissue around the hematomas was retained by decapitation method. The water content of brain tissue was measured by dry and wet specific gravity method, the expression of AQP4 was measured by immunohistochemistry, and the activity of SOD was measured by xanthine oxidase method. Results: (1) the water content of brain tissue in cerebral hemorrhage group and hyperbaric oxygen treatment group was higher than that in normal group (P 0.05), and the water content in brain tissue in hyperbaric oxygen treatment group was lower than that in cerebral hemorrhage group (P 0.05). The water content of brain tissue in hyperbaric oxygen treatment group was lower than that in 2 weeks at 1 week, 3 weeks and 4 weeks (P 0.05). There was no significant difference in the expression of AQP4 between the three groups (P 0.05). (2). The expression of AQP4 in brain tissue of cerebral hemorrhage group and hyperbaric oxygen treatment group was higher than that of normal group at 1 < 3 weeks (P 0.05). The expression of AQP4 in brain tissue of hyperbaric oxygen treatment group was lower than that of cerebral hemorrhage group at 1 < 3 weeks (P 0.05). The expression of SOD in brain tissue of hyperbaric oxygen treatment group was lower than that of normal group at the first and third week (P 0.05). (3). The expression of SOD in cerebral hemorrhage group and hyperbaric oxygen treatment group was higher than that in normal group at 1 and 3 weeks (P 0.05). The expression of SOD in hyperbaric oxygen treatment group was higher than that in cerebral hemorrhage group (P 0.05). The expression of SOD in hyperbaric oxygen treatment group was higher than that in 4 weeks in 1 week, 2 weeks and 3 weeks (P 0.05), and there was no difference among the three groups (P 0.05). Conclusion: hyperbaric oxygen therapy can down-regulate the expression of AQP4 and reduce the water content of brain tissue around hematomas and up-regulate the expression of SOD. Continuous hyperbaric oxygen therapy is superior to other courses of treatment for 4 weeks.
【作者单位】：河北医科大学第二医院康复医学二科(高压氧治疗室);
【分类号】：R743.34

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