高级糖基化终末产物受体下调对高迁移率族蛋白B1表达及移植瘤体积的影响

发布时间：2019-06-13 18:54

【摘要】：目的探究高级糖基化终末产物受体(RAGE)下调对胶质瘤细胞系中高迁移率族蛋白B1(HMGB1)的表达及裸鼠移植瘤体积变化的影响。方法用免疫组织化学法,蛋白印迹观察胶质瘤LN229细胞系(分为对照组和实验组)中HMGB1的表达,对照组用生理盐水处理,实验组用RAGE受体阻断剂FPS-ZM1处理,用同样方法检测上述处理后细胞系中HMGB1蛋白的表达水平,其表达的差异采用两独立样本t检验;选取30只Nu/Nu裸鼠并随机分为2组,分别于裸鼠左侧背部相同部位注射上述2种细胞系,于注射后连续6周,共计6次测量裸鼠背部移植瘤体积大小,体积变化的差异采用重复测量资料的方差分析。结果 HMGB1主要表达于细胞核和细胞质中,与对照组细胞系相比,实验组细胞系中HMGB1蛋白表达水平下降(P0.05),接种对照组的裸鼠移植瘤体积增长速度明显快于实验组(P0.05),差异存在统计学意义。结论 HMGB1蛋白可能通过RAGE受体参与胶质瘤的生长与侵袭,RAGE受体阻滞剂FPS-ZM1可以明显地降低HMGB1蛋白的表达,抑制移植瘤体积的增长,有望用于胶质瘤细胞凋亡的研究。
[Abstract]:Objective to investigate the effect of down-regulation of advanced glycosylation end product receptor (RAGE) on the expression of high mobility group protein B1 (HMGB1) in glioma cell line and the change of tumor volume in nude mice. Methods Immunohistochemical method and Western imprinting were used to observe the expression of HMGB1 in glioma LN229 cell line (divided into control group and experimental group). The control group was treated with saline and the experimental group was treated with RAGE receptor blocker FPS-ZM1. The expression level of HMGB1 protein in the above treated cell lines was detected by the same method. Thirty Nu/Nu nude mice were randomly divided into two groups. The two cell lines were injected into the same part of the left back of nude mice respectively. after 6 weeks of injection, the tumor volume of nude mice was measured for 6 times, and the difference of volume changes was analyzed by variance analysis of repeated measurement data. Results HMGB1 was mainly expressed in nucleus and cytoplasm. Compared with the control cell line, the expression level of HMGB1 protein in the experimental group decreased (P 0.05). The volume growth rate of the nude mice inoculated with the control group was significantly faster than that of the experimental group (P 0.05). Conclusion HMGB1 protein may be involved in the growth and invasion of glioma through RAGE receptor. RAGE receptor blocker FPS-ZM1 can significantly decrease the expression of HMGB1 protein and inhibit the growth of tumor volume, which is expected to be used in the study of apoptosis of glioma cells.
【作者单位】：郑州大学第五附属医院神经外科;
【基金】：河南省教育厅科学技术研究重点项目(编号:14A320078) 郑州市科技发展计划项目(编号:20150390)
【分类号】：R739.41

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