脂多糖损伤黑质导致迷走神经背核DR1和DR2表达变化

发布时间：2019-08-05 19:55

【摘要】：目的观察脂多糖(lipopolysaccharide,LPS)损伤大鼠黑质(substantia nigra,SN)后迷走神经背核(dorsal motor nucleus of the vagus,DMV)中酪氨酸羟化酶(tyrosine hydroxylase,TH)和多巴胺受体1(dopamine receptor 1,DR1)和多巴胺受体2(dopamine receptor 2,DR2)的表达变化。方法 30只SD大鼠根据随机数字表分为LPS大鼠组和对照组。LPS大鼠组双侧SN内注射LPS,对照组双侧SN内注射等体积的生理盐水。6周后进行转棒实验,检测胃排空能力,免疫荧光和免疫印迹检测SN内TH和DMV内TH、DR1和DR2的表达。结果经鉴定LPS组大鼠造模成功,胃排空能力降低,DMV中TH阳性细胞数量从(7±3)个增加到(12±2)个(P0.05),DR1阳性细胞数量从(28±5)个减少到(18±6)个(P0.05),DR2阳性细胞数量从(20±3)个增加到(33±4)个(P0.05)。背侧延髓内,TH蛋白表达水平从(0.16±0.02)增多到(0.43±0.04)(P0.05),DR1的蛋白表达水平从(0.78±0.05)下降至(0.36±0.03)(P0.05),DR2的蛋白表达水平从(0.43±0.03)增加至(0.58±0.07)(P0.05)。结论 LPS大鼠DMV中TH、DR1和DR2表达变化可能与帕金森病患者的胃排空障碍存在一定的联系。
[Abstract]:Aim to observe the expression of tyrosine hydroxylase,TH and dopamine receptor 1 (DR1) and dopamine receptor 2 (DR2) in dorsal vagus nucleus (dorsal motor nucleus of the vagus,DMV) after lipopolysaccharide (lipopolysaccharide,LPS) injury in substantia nigra (substantia nigra,SN) of rats. Methods 30 SD rats were randomly divided into LPS rat group and control group. LPs rats were injected into LPS, control group with the same volume of normal saline in bilateral SN. 6 weeks later, the gastric emptying ability was detected, and the expression of TH,DR1 and DR2 in SN and DMV was detected by immunofluorescence and immunoblotting. Results in LPS group, the model was established successfully, the gastric emptying ability was decreased, the number of TH positive cells in DMV increased from (7 卤3) to (12 卤2) (P 0.05), the number of DR1 positive cells decreased from (28 卤5) to (18 卤6) (P 0.05), and the number of DR2 positive cells increased from (20 卤3) to (33 卤4) (P 0.05). In the dorsal medulla oblongata, the expression of TH protein increased from (0.16 卤0.002) to (0.43 卤0.004), the protein expression of DR1 decreased from (0.78 卤0.005) to (0.36 卤0.003) (P 0.05), and the protein expression of DR2 increased from (0.43 卤0.03) to (0.58 卤0.07) (P 0.05). Conclusion the expression of TH,DR1 and DR2 in DMV of LPS rats may be related to gastric emptying disorder in patients with Parkinson's disease.
【作者单位】：河南省医用组织再生重点实验室;新乡医学院人体解剖学教研室;新乡医学院人体解剖学实验室;
【基金】：国家自然科学基金青年科学基金项目(31200897) 新乡医学院博士科研基金(XYBSKYZZ201501) 河南省医用组织再生重点实验室开放课题(KFKT16003)
【分类号】：R742.5

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