rno-miR-124-3p在大鼠急性持续性脑缺血过程中对MCT1表达的调控作用
发布时间：2021-08-10 16:53
第一部分 MCT1在急性持续性脑缺血大鼠脑组织中的表达变化目的:观察MCT1（monocarboxylate transporter 1,MCT1）在急性持续性脑缺血大鼠脑组织中的表达变化。方法:1.健康成年雄性SD大鼠,大鼠随机分为假手术组（n=10）和模型组（n=50）,模型组又分为5个亚组,分别于造模成功后1 h,3 h,6 h,12 h,24 h取材。2.运用线栓法制作持续性大脑中动脉栓塞（permanent middle cerebral artery occlusion,p MCAO）大鼠脑缺血模型,并进行行为学观察。3.运用TTC染色法,观察缺血后不同时段脑组织的缺血情况变化。4.运用免疫荧光技术,激光共聚焦检测MCT1在大鼠脑组织中的表达;运用荧光双标法检测MCT1与星形胶质细胞标记物GFAP（Glial fibrillary acidic protein,GFAP）的共表达情况。5.运用Western blot技术,检测脑缺血后不同时段大鼠脑组织MCT1蛋白表达变化。结果:1.构建p MCAO模型后大鼠的行为学变化如下:1 h内大鼠尚未苏醒,3 h后大鼠均已苏醒,提起...
【文章来源】：重庆医科大学重庆市
【文章页数】：94 页
【学位级别】：博士
【部分图文】：
大鼠pMCAO模型制作和模型成功后大鼠的症状及取材所见
图 1-2 尼氏染色检测 pMCAO-24 h 后脑组织神经元的变化。A1 和 A2 示大脑皮质顶叶附近皮质的尼氏染色结果。B1 和 B2 示大脑皮质靠近侧脑室的尼氏染色结果。C1 和 C2 示大脑皮质颅底附近皮质的尼氏染色结果。在梗塞侧，上述三个区域都仅能看到一些破碎的染色较浅的细胞碎片，不能看到神经元的形态；在对照侧，则可清楚地看到染成紫蓝色的神经元及 Nisslbody。Fig. 1-2 Nissl staining detecting of the changes of neurons in the brain after 24 h of pMCAO.A1 and A2 show the nissl staining results of the cortex near the parietal cortex. B1 and B2showed the results of nissl staining of cerebral cortex near lateral ventricle.C1 and C2 show theresults of nissl staining in the cortex near the cerebral cortex base.In the above three areas onthe infarct side, only some fragmented and lightly stained cell fragments could be seen, but themorphology of neurons could not be seen.On the control side, the purple-blue stained neuronsand Nissl body were clearly seen.
图 1-3 假手术及缺血后 1 h、3 h、6 h、12 h、24 h 时的 TTC 染色。缺血区显示为白色。在假手术组大鼠的脑组织内没有苍白色缺血灶出现；从缺血 1 h 开始，皮质部分可观察到缺血区，随着缺血时延长至 24 h，缺血区域面积逐渐增大至梗塞侧全部脑区。Fig. 1-3 TTC staining at 1 h, 3 h, 6 h, 12 h, and 24 h after ischemia and those of sham group. Theischemic areas are shown in white. No pale ischemic lesions were found in the brain tissue of thesham group. Ischemic regions can be observed in the cortex from after 1 h ischemia, and theischemic area gradually increased to the entire cerebral area on the infarct side upto after 24 hischemia.
本文编号：3334434
【文章来源】：重庆医科大学重庆市
【文章页数】：94 页
【学位级别】：博士
【部分图文】：
大鼠pMCAO模型制作和模型成功后大鼠的症状及取材所见
图 1-2 尼氏染色检测 pMCAO-24 h 后脑组织神经元的变化。A1 和 A2 示大脑皮质顶叶附近皮质的尼氏染色结果。B1 和 B2 示大脑皮质靠近侧脑室的尼氏染色结果。C1 和 C2 示大脑皮质颅底附近皮质的尼氏染色结果。在梗塞侧，上述三个区域都仅能看到一些破碎的染色较浅的细胞碎片，不能看到神经元的形态；在对照侧，则可清楚地看到染成紫蓝色的神经元及 Nisslbody。Fig. 1-2 Nissl staining detecting of the changes of neurons in the brain after 24 h of pMCAO.A1 and A2 show the nissl staining results of the cortex near the parietal cortex. B1 and B2showed the results of nissl staining of cerebral cortex near lateral ventricle.C1 and C2 show theresults of nissl staining in the cortex near the cerebral cortex base.In the above three areas onthe infarct side, only some fragmented and lightly stained cell fragments could be seen, but themorphology of neurons could not be seen.On the control side, the purple-blue stained neuronsand Nissl body were clearly seen.
图 1-3 假手术及缺血后 1 h、3 h、6 h、12 h、24 h 时的 TTC 染色。缺血区显示为白色。在假手术组大鼠的脑组织内没有苍白色缺血灶出现；从缺血 1 h 开始，皮质部分可观察到缺血区，随着缺血时延长至 24 h，缺血区域面积逐渐增大至梗塞侧全部脑区。Fig. 1-3 TTC staining at 1 h, 3 h, 6 h, 12 h, and 24 h after ischemia and those of sham group. Theischemic areas are shown in white. No pale ischemic lesions were found in the brain tissue of thesham group. Ischemic regions can be observed in the cortex from after 1 h ischemia, and theischemic area gradually increased to the entire cerebral area on the infarct side upto after 24 hischemia.
本文编号：3334434
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