烟酰胺腺嘌呤二核苷酸对实验性自身免疫性脑脊髓炎小鼠SIRT3/AMPK/mTOR通路的影响
发布时间：2022-02-10 04:37
目的:研究烟酰胺腺嘌呤二核苷酸（Nicotinamide adenine dinucleot-ide,NAD+）对实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis,EAE）小鼠脑组织中沉默信息调节因子3（Silent information regulator of transcription 3,SIRT3）表达的影响,探究NAD+对EAE小鼠发挥治疗作用可能的通路及机制,为临床多发性硬化的治疗寻找新的思路。方法:1.选用C57BL/6雌性小鼠（周龄8-10周、体重18-20g）,以MOG35-55作为免疫抗原,辅以完全弗氏佐剂、结核菌素充分乳化,使结核杆菌H37Ra的终浓度为4mg/ml,于小鼠背部脊柱两旁皮下注射,并分别于免疫的当天（免疫当天为第0天）及第2天,分两次腹腔注射0.5ml百日咳毒素（PTX）,构建EAE小鼠模型。2.将实验小鼠随机分为正常对照组（Control组）、EAE模型组、NAD+治疗组,每组8只。免疫后的第1天起...
【文章来源】：河北医科大学河北省
【文章页数】：49 页
【学位级别】：硕士
【部分图文】：
NAD+降低EAE小鼠的Weaver’s评分Fig.1NAD+reducedWeaver’sscoresofmodel(EAE)mice.
图 2 NAD+减轻 EAE 小鼠中枢神经系统炎性细胞浸润及脱髓鞘程度Fig.2 NAD+mitigates inflammation and demyelination in EAE mice.HE analysis of the spinal cord shows the infiltration of inflammatory ce white matter (a 50×, b 200×). The arrows indicate the inflammat. (B) LFB analysis of the spinal cord shows the areas of intact mye) and demyelination (white) (a 50×, b 200×). The arrows indicate yelination in the spinal cord. (C) HE analysis of the brain shows tration of inflammatory cells in the perivascular (200×). The arroate the inflammatory cells. (D) LFB analysis of the brain shows the artact myelin (blue) and demyelination (white) (50×). The arrows indicdemyelination in the spinal cord. (E) The mean pathological scores mmation and demyelination in Control、PBS-treated EAE mice +-treated mice were measured by semi-quantitative analysis. * P <0
行石蜡包埋切片后，进行 SIRT3 免疫组织化学染色。结果如图 3A 所E 组与 Control 组相比 SIRT3 阳性细胞表达数目明显减少，而 NAT3 阳性细胞表达数较 EAE 组显著增加。统计分析可得（图 3B）：与 Control 组阳性细胞数分别为 2.83±1.169、16.17±4.708，差异具学意义（P<0.01）。NAD+组阳性细胞数为 23.83±4.997，与 EAE 组相异具有统计学意义（P<0.01）。AControl EAE NAD+
本文编号：3618263
【文章来源】：河北医科大学河北省
【文章页数】：49 页
【学位级别】：硕士
【部分图文】：
NAD+降低EAE小鼠的Weaver’s评分Fig.1NAD+reducedWeaver’sscoresofmodel(EAE)mice.
图 2 NAD+减轻 EAE 小鼠中枢神经系统炎性细胞浸润及脱髓鞘程度Fig.2 NAD+mitigates inflammation and demyelination in EAE mice.HE analysis of the spinal cord shows the infiltration of inflammatory ce white matter (a 50×, b 200×). The arrows indicate the inflammat. (B) LFB analysis of the spinal cord shows the areas of intact mye) and demyelination (white) (a 50×, b 200×). The arrows indicate yelination in the spinal cord. (C) HE analysis of the brain shows tration of inflammatory cells in the perivascular (200×). The arroate the inflammatory cells. (D) LFB analysis of the brain shows the artact myelin (blue) and demyelination (white) (50×). The arrows indicdemyelination in the spinal cord. (E) The mean pathological scores mmation and demyelination in Control、PBS-treated EAE mice +-treated mice were measured by semi-quantitative analysis. * P <0
行石蜡包埋切片后，进行 SIRT3 免疫组织化学染色。结果如图 3A 所E 组与 Control 组相比 SIRT3 阳性细胞表达数目明显减少，而 NAT3 阳性细胞表达数较 EAE 组显著增加。统计分析可得（图 3B）：与 Control 组阳性细胞数分别为 2.83±1.169、16.17±4.708，差异具学意义（P<0.01）。NAD+组阳性细胞数为 23.83±4.997，与 EAE 组相异具有统计学意义（P<0.01）。AControl EAE NAD+
本文编号：3618263
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