联合应用基质细胞衍生因子1、辛伐他汀及骨胶原支架进行体内异位成骨

发布时间：2017-12-28 06:19

本文关键词：联合应用基质细胞衍生因子1、辛伐他汀及骨胶原支架进行体内异位成骨　出处：《北京大学学报(医学版)》2015年01期 　论文类型：期刊论文

更多相关文章： 趋化因子CXCL 辛伐他汀 胶原 骨生成

【摘要】：目的:探讨使用小鼠基质细胞衍生因子1(murine stromal cell-derived factor 1,m SDF-1)结合辛伐他汀(simvastatin,SIM)以及骨胶原支架(Bio-Oss湟)构建无外加种子细胞的组织工程化骨的可行性,并检验其体内异位成骨的效果。方法:将32只ICR小鼠随机分为4组,每组8只,于小鼠颅部做皮肤切口,各组小鼠分别植入:(1)1∶50(体积比)的二甲基亚砜(dimethyl sulfoxide,DMSO)/磷酸盐缓冲液(phosphate-buffered saline,PBS)混合液+骨胶原支架(空白对照组);(2)10-3mol/L SIM溶液+骨胶原支架(SIM组);(3)200 mg/L m SDF-1溶液+骨胶原支架(m SDF-1组);(4)10-3mol/L SIM+200 mg/L m SDF-1溶液+骨胶原支架(SIM+m SDF-1组)。植入1周后,连续2 d,每天分别在支架局部注射上述各组相应的溶液50μL。饲养6周后,取出支架及其周围组织,通过软X射线投射成像及灰度测定、HE染色、免疫组织化学染色的方法,定性及定量观察其成骨效果。结果:SIM+m SDF-1组软X射线灰度值[(421 836.5±65 425.7)像素]明显高于空白对照组[(153 345.6±45 222.2)像素,P0.01]、SIM组[(158 119.2±100 284.2)像素,P0.01]以及m SDF-1组[(255 529.5±152 142.4)像素,P0.05];在SIM+m SDF-1组内可见明显的骨桥蛋白和骨钙素的表达;SIM+m SDF-1组血管丛密度[(46±8)条/mm2]明显高于空白对照组[(23±7)条/mm2,P0.01]和SIM组[(24±6)条/mm2,P0.01]。结论:使用m SDF-1结合SIM以及骨胶原支架构建的无外加种子细胞的组织工程化骨可于小鼠颅部皮下异位成骨。
[Abstract]:Objective: To investigate the use of mouse stromal cell derived factor 1 (murine stromal cell-derived factor 1, m SDF-1) combined with simvastatin (simvastatin, SIM) and collagen scaffold (Bio-Oss Huang) plus the feasibility of construction of tissue engineered bone seed cells, and examine the effect of ectopic osteogenesis in vivo. Methods: 32 ICR mice were randomly divided into 4 groups, 8 rats in each group, in the mouse cranial skin incision, the mice were implanted with 1 (1): 50 (volume ratio) of two dimethyl sulfoxide (dimethyl sulfoxide, DMSO) / phosphate buffer (phosphate-buffered saline, PBS) mixed liquid + collagen scaffold (control group); (2) 10-3mol/L SIM solution + collagen scaffold (SIM group); (3) 200 mg/L m SDF-1 solution + collagen scaffold (M group SDF-1); (4) 10-3mol/L SIM+200 mg/L m SDF-1 solution + collagen scaffold (SIM+m group SDF-1). After 1 weeks of implantation, 2 D were continuous, and the corresponding solution 50 u L of the above groups were injected each day respectively. After 6 weeks of feeding, the scaffolds and surrounding tissues were taken out. Qualitative and quantitative osteogenic effects were detected by soft X ray projection imaging, gray scale determination, HE staining and immunohistochemical staining. Results: SIM+m group SDF-1 soft X ray gray value [(421836.5 + 65425.7) pixels] was significantly higher than the control group [(153345.6 + 45222.2) P0.01] pixels, and SIM Group [(158119.2 + 100284.2) P0.01] and M pixels, SDF-1 Group [(255529.5 + 152142.4) P0.05]; visible pixel, osteopontin and osteocalcin the expression of SIM+m in SDF-1 group; SIM+m SDF-1 group vascular plexus [(46 + 8) density of /mm2] was significantly higher than the control group [(23 + 7) /mm2, P0.01] and SIM Group [(24 + 6) /mm2, P0.01]. Conclusion: the tissue engineered bone without seed cells using M SDF-1 combined with SIM and bone collagen scaffold can be subcutaneous ectopic osteogenesis in the skull of mice.
【作者单位】：北京大学口腔医学院·口腔医院第三门诊部;北京大学口腔医学院·口腔医院修复科;口腔数字化医疗技术和材料国家工程实验室;
【基金】：国家自然科学基金(81170937,81400484) 教育部新世纪优秀人才支持计划(NCET-11-0026)资助~~
【分类号】：R318.08
【正文快照】： 构建组织工程化骨一般需要三个要素:可成骨向分化的种子细胞、促成骨向分化的诱导因子和起支撑以及引导骨形成的三维支架。但是,获得足量的种子细胞需要经历长期的体外扩增、传代,增加了细胞污染、生物安全性受到影响等风险,而且其周期长、费用高等,这些缺点都影响该技术的临

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