重组人类骨形态发生蛋白2复合纤维蛋白凝胶与血管内皮生长因子进行的脊柱融合

发布时间：2017-12-28 20:12

本文关键词：重组人类骨形态发生蛋白2复合纤维蛋白凝胶与血管内皮生长因子进行的脊柱融合　出处：《中国组织工程研究》2016年25期 　论文类型：期刊论文

【摘要】：背景:骨形态发生蛋白2具有骨诱导作用,血管内皮生长因子对骨形成及修复起正性调节作用,那么二者用于骨融合的效果如何呢?目的:观察重组人类血管内皮生长因子165和重组人类骨形态发生蛋白2复合纤维蛋白凝胶进行脊柱横突间融合的效果。方法:将30只日本大耳白兔随机分3组,每组10只,实验组于L5-6横突间置入重组人类血管内皮生长因子165/重组人类骨形态发生蛋白2/纤维蛋白凝胶复合材料,对照组于L5-6横突间置入重组人类骨形态发生蛋白2/纤维蛋白凝胶复合材料,空白对照组于L5-6横突间置入纤维蛋白凝胶材料。置入后6周,进行影像学、生物力学与组织学检查。结果与结论:实验组、对照组横突间成骨融合,且实验组成骨效果优于对照组,空白对照组无成骨。实验组成骨融合强度高于对照组(P0.01)。实验组成骨融合标本可见大量软骨细胞、骨细胞、成熟骨组织与胶原组织,对照组成骨融合标本可见大量软骨细胞、骨细胞及骨组织。结果表明,重组人类血管内皮生长因子165和重组人类骨形态发生蛋白2联合应用有助于促进成骨融合。
[Abstract]:Background: bone morphogenetic protein 2 with bone induction, vascular endothelial growth factor on bone formation and repair plays a positive regulatory role, then the two for how bone fusion effect? Objective: To observe the effect of recombinant human vascular endothelial growth factor 165 and recombinant human bone morphogenetic protein 2 fibrin gel spine transverse the effect of process fusion. Methods: 30 rabbits were randomly divided into 3 groups, 10 rats in each group, the experimental group L5-6 intertransverse implantation of recombinant human vascular endothelial growth factor 165/ recombinant human bone morphogenetic protein 2/ fibrin gel composite materials, in the control group L5-6 intertransverse implantation of recombinant human bone morphogenetic protein 2/ protein fiber composite gel, control group L5-6 intertransverse placement of fibrin gel materials. 6 weeks after implantation, imaging, biomechanics and histological examination were carried out. Results and conclusion: in the experimental group and the control group, the bone fusion was formed between the transverse processes, and the experimental group was better than the control group, and the blank control group had no osteogenesis. The intensity of bone fusion in the experiment was higher than that of the control group (P0.01). A large number of chondrocytes, osteoblasts, mature bone tissues and collagen tissue were found in the bone fusion specimens, and a large number of chondrocytes, bone cells and bone tissue were found in the control group. The results showed that the combination of recombinant human vascular endothelial growth factor 165 and recombinant human bone morphogenetic protein 2 could help to promote osteogenic fusion.
【作者单位】：沈阳市红十字会医院骨外科;中国医科大学附属第一医院骨外科;大连市第三人民医院骨外科;
【分类号】：R318.08
【正文快照】： 文章快速阅读:文题释义:纤维蛋白凝胶:是由纤维蛋白原与凝血酶反应后形成的立体网状结构、低抗原生物大分子材料,可塑性好,其在体内逐渐降解过程为药物缓慢均匀释放提供了良好条件,立体网状结构也允许新生毛细血管长入。以往的实验表明,纤维蛋白凝胶是骨形态发生蛋白2的良好载

【相似文献】