壳聚糖与海藻酸钙双相陶瓷骨支架的机械性能及细胞相容性

发布时间：2018-01-01 09:08

本文关键词：壳聚糖与海藻酸钙双相陶瓷骨支架的机械性能及细胞相容性　出处：《中国组织工程研究》2016年08期 　论文类型：期刊论文

【摘要】：背景:由羟基磷灰石和少量β-磷酸三钙构成的双相陶瓷骨具有良好的细胞相容性,但机械性能较差。目的:构建壳聚糖、海藻酸钠胶体与双相陶瓷骨复合支架材料,检测其机械性能和细胞相容性。方法:将不同浓度的壳聚糖(2%、4%、7%、10%)、海藻酸钠胶体(3%、4%、5%、7%)分别与双相陶瓷骨混合,制备壳聚糖/双相陶瓷骨支架及海藻酸钙/双相陶瓷骨支架,检测其微观形态与结构、凝固时间、抗溶解性能、抗剪切力、抗压缩力及细胞相容性。结果与结论:1凝固时间:随着胶体浓度的增大,两种复合支架的初凝时间和终凝时间均有所延长。2扫描电镜:材料均呈多孔状的微观结构,孔隙大小不一。3抗溶解性能:3%、4%、5%、7%的海藻酸钙/双相陶瓷骨支架和7%、10%的壳聚糖/双相陶瓷骨支架,在液体环境中抗溶解性能良好。4力学强度:随着胶体浓度的增大,壳聚糖/双相陶瓷骨支架的抗剪切力、抗压缩力均逐渐增强;海藻酸钙/双相陶瓷骨支架抗剪切力、抗压缩力均逐渐增强,但至7%浓度时,抗剪切力、抗压缩力均降低。5细胞相容性:壳聚糖/双相陶瓷骨支架的细胞毒性为0至1级,海藻酸钙/双相陶瓷骨支架的细胞毒性为2至3级。6结果表明,壳聚糖/双相陶瓷骨支架具备良好的机械性能及细胞相容性。
[Abstract]:Background: the biphasic ceramic bone composed of hydroxyapatite and a small amount of 尾 -tricalcium phosphate has good cytocompatibility but poor mechanical properties. Objective: to construct chitosan. The mechanical properties and cytocompatibility of sodium alginate colloid and biphasic ceramic bone composite scaffold were tested. The chitosan / biphasic ceramic bone scaffolds and calcium alginate / biphasic ceramic bone scaffolds were prepared, and the microstructure, solidification time and solubility resistance of the scaffolds were measured. Results and conclusion Solidification time of 1: 1: with the increase of colloid concentration. The initial setting time and the final setting time of the two kinds of composite scaffolds were both prolonged. 2 scanning electron microscope: the materials were all porous microstructures, and the pore size varied from 3 to 3%. The dissolution resistance of the composite scaffolds was 5%. 7% calcium alginate / biphasic ceramic bone scaffold and 710% chitosan / biphasic ceramic bone scaffold have good anti-dissolution properties in liquid environment. 4 mechanical strength is good: with the increase of colloid concentration. The shear and compression resistance of chitosan / biphasic ceramic scaffold increased gradually. The shear and compression resistance of calcium alginate / biphasic ceramic bone scaffold increased gradually, but when the concentration reached 7%, the shear resistance was increased. The cytotoxicity of chitosan / biphasic ceramic bone scaffold was 0 to 1 grade, and the cytotoxicity of calcium alginate / biphasic ceramic bone scaffold was 2 to 3 grades 6. 6. Chitosan / biphasic ceramic bone scaffold has good mechanical properties and cytocompatibility.
【作者单位】：新疆医科大学第一附属医院口腔修复科;
【基金】：新疆维吾尔自治区科技厅自然科学基金项目(2014211C037):三维打印蛋白复合型陶瓷化骨及颌骨缺损修复能力的实验研究~~
【分类号】：R318.08
【正文快照】： 文章快速阅读:文题释义:双相陶瓷骨:由大量羟基磷灰石和少量β-磷酸三钙经低温锻烧等工艺处理制得,具有天然骨小梁结构及多孔网状结构,不仅可为牙髓细胞重建起支架作用,还为矿化组织形成提供无机离子,具备优良的生物相容性,但机械力学性能不佳。壳聚糖:由2-氨基-2-脱氧-β-D-

【相似文献】