国产多孔钽复合骨形态发生蛋白7植入兔竖脊肌内的生物相容性

发布时间：2018-01-06 05:18

本文关键词：国产多孔钽复合骨形态发生蛋白7植入兔竖脊肌内的生物相容性　出处：《中国组织工程研究》2016年16期 　论文类型：期刊论文

【摘要】：背景:骨形态发生蛋白7在体内可诱导骨及软骨形成,并可诱导肌肉中和血管周围的间充质细胞分化为软骨和骨细胞,有促进软骨和骨形成的作用。目的:观察多孔钽复合骨形态发生蛋白7植入兔竖脊肌后,钽-肌肉界面纤维包膜结构、肌肉与小血管向多孔钽内生性生长及异位成骨的能力。方法:在新西兰大白兔左右两侧竖脊肌内分别植入复合骨形态发生蛋白7的多孔钽片(实验组)和多孔钽片(对照组),植入后2,4,8周,取钽片及其周围0.5 cm肌肉组织,进行扫描电镜、苏木精-伊红染色、Masson染色及硬组织切片观察。结果与结论:①苏木精-伊红染色:两组材料周围均有纤维性包膜形成,随时间延长,纤维性包膜逐渐由疏松变致密,厚度也逐渐变薄,材料与肌肉交界面无明显炎症反应。两组间纤维包膜厚度比较差异无显著性意义。②扫描电镜:植入2周时,两组多孔钽表面可见少量肌肉及胶原纤维逐渐长入孔隙内部,部分胶原纤维附着于孔壁;植入8周时,多孔钽孔隙内充满了肌腱纤维,纤维与孔壁结合紧密,两组间无明显差异。③硬组织切片:植入2周时,两组多孔钽孔隙均内有少量成纤维细胞及肌纤维长入,实验组材料孔隙内可见有新生小血管长入;植入8周时,两组多孔钽表面和孔隙内均长满呈条索状交错排列的肌纤维,小血管及细胞成分减少,钽-肌肉紧密融合。④Masson染色:植入8周时,实验组钽-肌肉界面边缘处肌肉内可见大量间充质细胞、骨胶原及软骨基质形成,以及少量新生的软骨化骨,对照组未见软骨化骨。⑤结果表明,复合骨形态发生蛋白7的多孔钽具有良好生物相容性及诱导成骨作用。
[Abstract]:Background: bone morphogenetic protein 7 in vivo can induce bone and cartilage formation, and can induce muscle and surrounding vascular mesenchymal cells to differentiate into cartilage cells and bone cells, promote cartilage and bone formation. Objective: To observe the effect of porous tantalum combined with bone morphogenetic protein 7 rabbit erector spinae, TA muscle fiber interface envelope structure, muscle and small blood vessels to grow within the porous tantalum and ectopic osteogenesis ability is in New Zealand rabbits. Methods: the left and right sides of the erector spinae muscles was implanted with bone morphogenetic protein 7 porous tantalum (experimental group) and porous tantalum (control group), 2,4,8 weeks after implantation take, tantalum and around 0.5 cm muscle tissue by scanning electron microscope, hematoxylin eosin staining, Masson staining and hard tissue. Results and conclusion: hematoxylin eosin staining: two groups were formed around the material fibrous capsule, with the extension of time, Fibrous capsule gradually from loose density, thickness gradually thinning, material and muscle interface without obvious inflammatory reaction. No significant difference between the two groups. The fiber coating thickness difference scanning electron microscope: after 2 weeks of implantation, two groups of porous tantalum surface shows a small amount of muscle and collagen fiber gradually grow into the inner pore. Some collagen fibers attached to the hole wall; 8 weeks after implantation, the porous tantalum is filled with the tendon fibers, the combination between the fiber and the hole wall, no obvious difference between the two groups. The hard tissue slices: at 2 weeks after operation, two groups of porous tantalum porosity are within a small amount of fibroblasts and muscle fiber ingrowth visible small vessels grow into the material porosity within the experimental group; 8 weeks after implantation, two groups of porous tantalum surface and the pores are covered with a streak of staggered muscle fibers, small blood vessels and cells reduced, TA muscle tightness fusion. The Masson staining: 8 weeks of implantation When the experimental group TA muscle interface edge in muscle showed a large amount of mesenchymal cells, collagen and cartilage matrix formation, as well as a small amount of new cartilage bone, not in the control group. The results showed that the cartilage bone, bone morphogenetic protein 7 porous tantalum has good biocompatibility and osteogenesis role.

【作者单位】：唐山市第二医院关节一科;华北理工大学基础医学院人体解剖学系;唐山市第二医院创伤二科;华北理工大学附属医院骨科;华北理工大学医学中心实验室;
【基金】：国家科技部科技支撑课题资助项目(2012BAE06B03) 河北省科技支撑资助项目(16277776D) 河北省医学科学研究重点课题计划项目(20160225) 华北理工大学大学博士科研启动基金资助项目~~
【分类号】：R318.08;R687
【正文快照】： 0 引言Introduction 多孔金属钽是一种新型的骨科植入材料,呈多重的十二面体排列,具有纵横交错、相互连通的微孔结构,这使其具有孔隙率高、弹性模量低及表面摩擦系数高等物理特性。多孔钽金属无明显生物毒性,抗腐蚀能力强,具有良好的骨传导性,是理想的骨缺损修复材料[1]。鉴

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