集成化荧光激活液滴分选系统研究

发布时间：2018-01-15 13:00

本文关键词：集成化荧光激活液滴分选系统研究　出处：《中国生物医学工程学报》2016年03期 　论文类型：期刊论文

【摘要】：高通量的荧光激活液滴分选技术在大规模生化试验中能够显著降低实验成本,缩短实验时间,因此在微生物菌株筛选、新药研发、高通量单细胞研究等众多领域具有广泛的应用。然而,目前使用的荧光激活液滴分选系统大多基于光学试验器件搭建,系统的灵活性、稳定性相对较差,搭建的技术门槛较高,限制了该技术在生命科学研究等领域的推广应用。设计一种集成化的荧光激活液滴分选系统,通过特定光路设计,系统所需的荧光激发和探测光路实现集成化、模块化封装,荧光激发和探测功能模块缩小到160 mm×143 mm×54 mm,大幅缩减系统体积。可以通过给普通显微镜增加功能模块的方式,快速实现分选系统的搭建,从而提高荧光激活液滴分选技术的易用性。相比现有文献报道的荧光激活液滴分选系统,成本下降到1/5,体积减小为1/40,有利于该技术的工业化推广。
[Abstract]:The activation of fluorescent high-throughput droplet sorting technology can significantly reduce the cost of experiment in large-scale biochemical tests, shorten the test time, so screening microbial strains, in new drug research and development, has been widely used in many fields of high-throughput single cell research. However, the current use of fluorescent droplet sorting systems are activated optical devices based on test structures the flexibility of the system, the stability is relatively poor, build a high technical threshold, limiting the application of the technology in the field of life science research. The design of an integrated fluorescence activated droplet sorting system, through specific optical design, fluorescence excitation and detection system for the optical implementation of integrated, modular package the fluorescence excitation and detection function module, down to 160 mm * 143 mm * 54 mm, significantly reduce the system volume. By increasing the functional module for ordinary microscope, The implementation of sorting system can be achieved quickly, so as to improve the usability of fluorescence activated droplet sorting technology. Compared with the fluorescence activated droplet sorting system reported in the literature, the cost is reduced to 1/5 and the volume is reduced to 1/40, which is conducive to the industrial extension of the technology.

【作者单位】：北京航空航天大学生物与医学工程学院;
【分类号】：R318
【正文快照】： 引言微流控技术能够在很小的尺度上精细地操作流体的运动,配合特殊设计的芯片,能够在片上实现从样品的制备、孵育到反应产物的纯化、分析等一系列操作[1-2],因而催生了片上实验室的概念。该技术能够大大简化传统生化试验流程,降低实验试剂的消耗,提高试验的自动化程度,近年来

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