添加载银纳米二氧化钛光亮漆体外细胞毒性及其抗菌性的实验研究

发布时间：2018-01-16 16:16

本文关键词：添加载银纳米二氧化钛光亮漆体外细胞毒性及其抗菌性的实验研究　出处：《华西口腔医学杂志》2014年06期 　论文类型：期刊论文

【摘要】：目的研究添加载银纳米二氧化钛(Ag/TiO2)抗菌剂对软衬硅橡胶光亮漆体外细胞毒性及抗菌性能的影响。方法制备直径10 mm、厚度2 mm的Silagum-Comfort软衬硅橡胶圆形试样,随机分成6组,将Ag/TiO2按0、0.5%、1.0%、1.5%、2.0%、2.5%的重量比分别添加于光亮漆的基质和催化剂中,均匀搅拌后分别涂布于对应组试件表面一遍,制成含不同浓度抗菌剂的软衬硅橡胶试件;采用噻唑蓝比色法检测软衬硅橡胶试件对3T3细胞(小鼠胚胎成纤维细胞)的体外细胞毒性;使用粗糙度仪测量软衬硅橡胶试件表面粗糙度值(Ra);采用贴膜法检测软衬硅橡胶试件的体外抗白色假丝酵母菌性能。结果各实验组细胞毒性级别为0~2级。光亮漆的Ra值随着抗菌剂浓度的增加有所提高,但各浓度抗菌剂组与0组相比无统计学差异。随着抗菌剂浓度的增加,抗菌率也提高;抗菌剂浓度为2.0%和2.5%时,抗菌率已达到97.5%和96.5%。结论 Ag/TiO2光亮漆具有良好的抗菌效果,能有效改善软衬硅橡胶的抗菌性能。
[Abstract]:Objective to study the effect of Ag / TIO _ 2 (Ag / TIO _ 2) on cytotoxicity and antibacterial properties of soft lined silicone rubber bright paint in vitro. The circular Silagum-Comfort soft lining samples with thickness of 2 mm were randomly divided into 6 groups, and the Ag/TiO2 was 0. 5% and 1. 5%, 2. 0%. The weight ratio of 2.5% was added to the substrate and catalyst of the bright paint, and then evenly stirred and coated on the surface of the corresponding sample respectively, and the soft lined silicone rubber specimen containing different concentrations of antimicrobial agent was made. The cytotoxicity of soft lined silicone rubber specimens to 3T3 cells (mouse embryonic fibroblasts) was detected by thiazolyl blue colorimetry in vitro. The surface roughness value of soft lined silicone rubber specimen is measured by rough degree instrument. The in vitro resistance to Candida albicans of soft lined silicone rubber specimens was tested by the method of film sticking. Results the cytotoxic grade of each experimental group was 0 ~ 2. The Ra value of bright paint increased with the increase of antimicrobial concentration. However, there was no statistical difference between the two groups. With the increase of the concentration of antimicrobial agents, the antibacterial rate also increased. When the concentration of antimicrobial agent was 2.0% and 2.5, the antibacterial rate reached 97.5% and 96.5.Conclusion Ag/TiO2 bright paint has good antibacterial effect. It can effectively improve the antibacterial properties of soft-lined silicone rubber.
【作者单位】：南昌大学附属口腔医院综合科;南昌大学附属口腔医院修复科;江西省儿童医院药剂科;
【分类号】：R783.1
【正文快照】： 软衬硅橡胶材料因具有良好的理化性能和生物相容性,被广泛应用在活动义齿修复、阻塞器以及颌面赝复体中;但也存在着不足之处,如表面疏松多孔,难于清洁,易滋生霉菌(尤其是白色假丝酵母菌),引发义齿性口炎,并加速了材料的老化,降低材料性能[1]。对此,国内外学者[1-3]作了一系列

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