Ti6Al7Nb材料表面微观形貌对细胞黏附影响的实验研究

发布时间：2018-01-29 16:47

本文关键词： 钛合金表面形貌细胞黏附分形维数　出处：《哈尔滨工业大学》2012年硕士论文　论文类型：学位论文

【摘要】：钛及钛合金作为生物材料在医学方面已经得到了广泛的应用，特别是在整形外科、口腔外科及心血管外科等领域。钛合金材料作为骨科植入物的理想材料，必需具有优良的生物相容性，能够与人体的内环境形成良好的界面结合。生物材料的表面微观形貌是影响机体与植入材料表面相互作用的主要因素。对植入物的表面结构进行图案化设计可以提高生物材料在体内的相容性，从而促进骨整合作用。因此，对钛系生物医用材料的表面微观形貌进行全面的评价并开展细胞黏附、增殖及生物学形态的研究具有重要意义。本课题选用Ti6Al7Nb生物材料，通过砂纸抛光、机械磨削及喷砂处理获得不同微观形貌的表面，进行MC3T3成骨细胞的黏附情况、增殖能力及形态学观察研究。实验结果表明钛合金的表面微观形貌影响着成骨细胞的黏着、铺展及分裂生长，并且经过分析总结得到不同表面粗糙度和分形维数对细胞黏附、增殖及形态的影响规律。利用噻唑蓝法（MTT）和细胞计数法分别检测成骨细胞的黏附和增殖情况。从表面形貌的高度幅值角度指出：适合MC3T3细胞早期黏附的表面粗糙度为Ra=2.323±0.056μm、Rz=16.915±0.543μm；较光滑和较粗糙的表面均表现出偏低的细胞黏附率；光滑表面有利于成骨细胞的增殖。运用分形理论对钛合金表面形貌进行分形维数计算，指出表面形貌的不规则程度随分形维数的增加而增加。细胞实验结果表明：成骨细胞的黏附率和增殖率均随分形维数的增加而降低；在相同时期，抛光表面较磨削表面和喷砂表面更适合成骨细胞的铺展；随着培养时间的增加，培养孔内的成骨细胞由梭形间隙排列增殖为“铺路状”重叠生长，抛光和磨削表面的细胞由不规则多边形、长梭形逐渐生长为“鳞片状”并由大量丝状伪足相互连接汇合，细胞接触引导现象明显，，喷砂表面的细胞由条形逐渐趋于星射线状，多指板状伪足锚接于表面凹坑边缘或是跨越沟槽与其他细胞相互相接。
[Abstract]:Titanium and titanium alloys as biomaterials have been widely used in medicine, especially in plastic surgery, oral surgery and cardiovascular surgery. Titanium alloy materials as orthopaedic implants are ideal materials. Good biocompatibility is required. The micro-morphology of biomaterials is the main factor affecting the interaction between the body and the implant surface. The surface structure of implants can be patterned. Improving the compatibility of biomaterials in vivo. Therefore, it is of great significance to evaluate the surface morphology of titanium biomedical materials and to study cell adhesion, proliferation and biological morphology. In this paper, Ti6Al7Nb biomaterials were used to obtain different microcosmic surfaces by sandpaper polishing, mechanical grinding and sandblasting. The adhesion of MC3T3 osteoblasts was carried out. The results showed that the surface morphology of titanium alloy affected the adhesion, spread and growth of osteoblasts. The effects of different surface roughness and fractal dimension on cell adhesion, proliferation and morphology were analyzed and summarized. Thiazolyl blue method. The adhesion and proliferation of osteoblasts were detected by cell counting method. The surface roughness suitable for early adhesion of MC3T3 cells was Ra=2.323 卤0.056 渭 m. Rz=16.915 卤0.543 渭 m; The smooth and rough surfaces showed low cell adhesion. Smooth surface is conducive to the proliferation of osteoblasts. Fractal theory is used to calculate the fractal dimension of titanium alloy surface morphology. It was pointed out that the irregular degree of surface morphology increased with the increase of fractal dimension. The results of cell experiment showed that the adhesion rate and proliferation rate of osteoblasts decreased with the increase of fractal dimension. In the same period, the polished surface is more suitable for osteoblast spreading than the grinding surface and the sand blasting surface. With the increase of culture time, the osteoblasts in the culture holes were arranged and proliferated from fusiform spaces to "paving" overlapping growth, and the cells on the polished and ground surfaces were irregular polygons. The long fusiform gradually grows into "scale" and is connected and conjuncted by a large number of filamentous pseudopodia, the cell contact and leading phenomenon is obvious, and the cells on the sand blasting surface gradually tend to star-ray shape from the stripe shape. Multiple-finger plate-shaped pseudopod anchors are attached to the edge of the surface pit or across grooves to connect with other cells.
【学位授予单位】：哈尔滨工业大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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