骨生物材料复合骨髓间充质干细胞异位成骨修复肋骨大段缺损

发布时间：2018-01-30 01:33

本文关键词： 藻酸盐间质干细胞组织工程生物材料骨生物材料海藻酸钠间充质干细胞组织工程骨肋骨大段缺损　出处：《中国组织工程研究》2017年02期 　论文类型：期刊论文

【摘要】：背景:组织工程生物材料具有与自体组织相似的结构与功能。目的:观察骨生物材料复合大鼠骨髓间充质干细胞异位成骨治疗肋骨大段缺损的效果。方法:取Wistar大鼠50只,制备右侧大段肋骨缺损模型,随机分为2组,对照组于骨缺损处置入氯化钙-藻酸钠凝胶,实验组于骨缺损处植入氯化钙-藻酸钠-骨髓间充质干细胞复合物。植入后2,4,8周,进行胸部X射线及骨缺损处组织形态学观察。结果与结论:1X射线观察结果:植入后2周,实验组骨缺损区无明显变化;植入后4周,缺损部位开始出现骨质痕迹;植入后8周,缺损部位大部分充满骨质,缺损两端逐渐相接。对照组缺损部位始终未见明显骨质修复,缺损两端逐渐封闭并硬化;2骨缺损处组织形态学观察结果:植入后2周,实验组材料腔隙中可见少量骨纤维组织,大量炎性细胞聚集,材料与骨质末端无连接;对照组骨缺部位可见大量炎性细胞,未见骨组织形成。植入后4周,实验组支架逐渐降解,可见大量新生骨质;对照组支架仅少量降解,缺损两端出现骨组织聚集。植入后8周,实验组支架大部分降解,可见大量骨组织、骨小梁等组织结构,缺损两端与再生骨质连接;对照组支架大部分降解,缺损两端出现骨硬化;3结果表明:骨生物材料复合大鼠骨髓间充质干细胞可促进肋骨大段缺损的修复。
[Abstract]:Background: tissue engineering biomaterials have the same structure and function as autologous tissues. Objective: to observe the effect of bone biomaterials combined with bone marrow mesenchymal stem cells (BMSCs) on heterotopic osteogenesis of rib defects. 50 Wistar rats were selected. The right large rib defect model was made and divided into two groups randomly. The control group was treated with calcium chloride sodium alginate gel in the treatment of bone defect. The experimental group was implanted with calcium chloride-sodium alginate-bone marrow mesenchymal stem cell complex at the site of bone defect. Results and conclusion the results showed that there was no significant change in bone defect area in the experimental group 2 weeks after implantation. 4 weeks after implantation, bone marks began to appear in the defect. At 8 weeks after implantation, most of the defects were filled with bone, and the two ends of the defects were gradually connected with each other. In the control group, there was no obvious bone repair at the defect site, and the two ends of the defect were gradually closed and sclerotic. 2Histomorphologic observation of bone defect: at 2 weeks after implantation, a small amount of bone fiber tissue was found in the space of the experimental group, and a large number of inflammatory cells gathered, and there was no connection between the material and the end of the bone. In the control group, a large number of inflammatory cells were found in the bone defect, but no bone tissue was formed. 4 weeks after implantation, the scaffolds in the experimental group were gradually degraded and a large number of new bone was observed. In the control group, there was only a small amount of degradation and bone tissue aggregation at the two ends of the defect. 8 weeks after implantation, most of the scaffolds in the experimental group were degraded, a large number of bone tissues and trabeculae were found, and the two ends of the defect were connected with the regenerated bone. In the control group, most of the scaffolds degraded and bone sclerosis appeared at both ends of the defect. The results showed that bone biomaterials combined with bone marrow mesenchymal stem cells could promote the repair of rib defect.
【作者单位】：郑州大学附属医院/南阳市中心医院胸外科;郑州大学附属医院/南阳市中心医院特需病房一病区;北京大学附属深圳医院胸外科;
【基金】：深圳市科技计划项目(JCYJ20140415162338820) 深圳市医疗卫生科研项目(201302068)~~
【分类号】：R318.08;R687
【正文快照】： 文章快速阅读:文题释义:海藻酸盐:是目前生物材料中一类研究较为热点的无机物,具有可塑性强、可降解、组织低排异度及X射线透视不显影的优势,因此选取海藻酸盐作为此次实验的主要生物无活性组织。为探究改善目前治疗大段肋骨缺损的手段,实验拟采取大鼠骨髓间充质干细胞为种子

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