尿液成分的近红外光谱分析原理及实现研究

发布时间：2018-02-01 05:38

本文关键词： 尿液近红外虚拟分析仪无试剂　出处：《重庆大学》2012年硕士论文　论文类型：学位论文

【摘要】：尿液检查是临床三大常规检查之一，其标本收集方便，尿液成分浓度的变化是反映泌尿生殖系统等人体代谢、脏器状态、病理变化的客观资料。然而，现行常用的借助生化化学方法的尿液分析，消耗大量试剂、费时费力、污染且价格昂贵。本文的目的在于利用目前活跃于分析化学领域中的近红外分析技术，构建一种快速、无试剂、无破坏的绿色尿液近红外检测分析系统。论文在简述现行几种尿液检查方法的原理与应用的基础上，阐述了近红外分析技术的原理和物理化学基础等定量分析基本理论应用于尿液成分分析检测中的可行性，采用近红外透射技术，进行尿液近红外检测分析仪的系统硬软件研制探索。硬件系统的实现上详述了整个光学系统、电路系统和机械系统的总体结构设计和各功能模块的实现过程。软件系统的实现基于Visual C++6.0开发平台，利用软件技术和虚拟仪器技术构建尿液近红外检测分析仪的软件系统，，实现对检测数据的显示、分析、打印、存储等虚拟仪器各功能模块。本研究用设计的系统样机进行了简单的测试实验，以验证系统的性能，为下一步实现完善的快速定量分析模型的建立奠定基础。近红外分析测试条件灵活，其检测结果包含的信息也十分丰富，本系统研究要达到临床实用仪器精度仍需要进行大量试验来优化分析模型，论文结尾对研究工作进行了总结和展望。
[Abstract]:Urine examination is one of the three routine clinical examinations, its specimen collection is convenient, the change of urine component concentration is the objective data of human metabolism, organ state and pathological changes. The commonly used urine analysis by biochemical methods consumes a lot of reagents, which is time-consuming, laborious, polluting and expensive. The purpose of this paper is to utilize the near infrared analysis technology which is active in the analytical chemistry field at present. A fast, reagent-free and non-destructive near infrared detection system for green urine was constructed. On the basis of brief introduction of the principle and application of several existing methods of urine examination. The feasibility of applying the basic theory of quantitative analysis, such as the principle of near infrared analysis technology and physicochemical basis, to the analysis and detection of urine composition is described. The near infrared transmission technique is adopted. The hardware and software of the urine near infrared detection analyzer are explored. The whole optical system is described in detail on the realization of the hardware system. The overall structure design of the circuit system and the mechanical system and the realization process of each functional module. The realization of the software system is based on the Visual C 6.0 development platform. The software system of urine near infrared detection analyzer is constructed by using software technology and virtual instrument technology. The function modules of virtual instrument such as displaying, analyzing, printing, storing and so on are realized. In order to verify the performance of the system, a simple test experiment is carried out with the designed system prototype, which lays a foundation for the establishment of a perfect fast quantitative analysis model in the next step. Near-infrared analysis test conditions are flexible, the detection results are also very rich in information, this system to achieve the accuracy of clinical instruments still need a large number of experiments to optimize the analysis model. At the end of the thesis, the research work is summarized and prospected.
【学位授予单位】：重庆大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：TH776

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