经皮种植体表面纳米二氧化硅抗菌涂层的研究

发布时间：2018-02-04 14:29

本文关键词： 纳米二氧化硅颗粒药物控释经皮种植体抗菌　出处：《实用口腔医学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:研究纯钛表面制备可降解载药二氧化硅纳米颗粒的抗菌性能。方法:通过非模版法制备可降解载庆大霉素的二氧化硅纳米颗粒,采用纳米粒径分析仪和扫描电镜对纳米粒进行特征分析;将载药纳米粒振荡交联至纯钛微弧氧化处理后形成的微米级形貌表面,用扫描电镜进行形态观测;将处理前后的样品与金黄色葡萄球菌共培养,体外抑菌圈法考察抗菌性能;对载药二氧化硅纳米颗粒的抗菌涂层进行体外药物释放实验。结果:载药纳米粒为直径(298.1±12.2)mm的圆球状;通过振荡交联后固定在纯钛微弧氧化层火山口样的结构内形成抗菌涂层;体外抑菌圈大小为(16.7±1.4)mm,而对照组未观测到抑菌圈;载药二氧化硅纳米颗粒的抗菌涂层可有效释放庆大霉素长达28 d。结论:基于可降解二氧化硅纳米粒制备的抗菌涂层可实现庆大霉素的缓控释放,并对金黄色葡萄菌的生长起到抑制作用,有望在今后应用于种植体经皮部位的设计。
[Abstract]:Objective: to study the antibacterial properties of biodegradable silica nanoparticles on titanium surface. Methods: the biodegradable gentamicin loaded silica nanoparticles were prepared by non-template method. Nano-particle size analyzer and scanning electron microscope were used to analyze the characteristics of nanoparticles. The micrometer surface formed by micro-arc oxidation of pure titanium was cross-linked by oscillatory cross-linking of drug-loaded nanoparticles, and morphology was observed by scanning electron microscope (SEM). The samples were co-cultured with Staphylococcus aureus before and after treatment, and the antibacterial properties were investigated by bacteriostatic circle method in vitro. In vitro drug release experiments were carried out on the antibacterial coating of drug-loaded silica nanoparticles. Results: the drug loaded nanoparticles were spherical in diameter (298.1 卤12.2 mm). The antibacterial coating was formed by oscillating crosslinking in the crater structure of pure titanium micro-arc oxidation layer. The bacteriostatic circle was 16.7 卤1.4mm in vitro, but not observed in the control group. The antibacterial coating of drug-loaded silica nanoparticles can effectively release gentamicin for 28 days. Conclusion: the antimicrobial coating based on degradable silica nanoparticles can realize the slow release of gentamicin. It is expected to be used in the design of the transdermal site of the implant in the future.
【作者单位】：军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔医学重点实验室第四军医大学口腔医院修复科;南京大学医学院附属口腔医院南京市口腔医院;军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔生物工程技术研究中心第四军医大学口腔医院口腔种植科;
【基金】：国家自然科学基金(编号:81300918)
【分类号】：R783.1
【正文快照】： \Ti/J/iniii^CTt7.^if-丁_rn士仲士7八々从ak 面缺损患者,目目!J利用经皮纯钛种植体固位的赝复体修复是很多患者最理想的治疗方式。但是,临床常用的种植体经皮段通常是光滑的,植人体内主要形成机械扣锁封闭,容易形成纤维包囊;并且由于经皮部位直接暴露于外界环境,在种植体植

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