生物可降解温度敏感性水凝胶用于PTH（1-34）的可控释放

发布时间：2018-02-04 17:45

  本文关键词： 温度敏感性 水凝胶 控制释放 甲状旁腺激素　出处：《吉林大学》2012年硕士论文　论文类型：学位论文

【摘要】：随着种植技术广泛应用于临床，，种植义齿作为“人类的第三副牙齿”已成为更多患者修复缺失牙的第一选择。牙槽骨质量和剩余牙槽骨体积是决定口腔种植体初期稳定性的关键性因素，但较多患者存在种植体周围骨量不足的问题。甲状旁腺激素1-34（即PTH1-34）优异的成骨作用引起了学者们的广泛关注，以往PTH1-34应用于促进种植体-骨愈合的实验研究多限于全身间断、小剂量给药方式，但是PTH1-34存在着易变性、半衰期短、价格昂贵等缺陷，因而研制控制其缓释的载体，并提高其生物利用率显得极为必要。环境敏感性高分子水凝胶是智能型水凝胶的一种，能够对外界刺激产生敏感响应。由于温度与人体生理环境密切相关，所以温度敏感性水凝胶的研究受到了广泛的关注。温度敏感性水凝胶可以随着环境温度的变化改变其自身的亲疏水性和凝胶网络的尺寸及体积，因而其作为药物载体时可随着温度的变化控制药物的释放速率。目前研究最广泛的的温度敏感性水凝胶是以聚N-异丙基丙烯酰胺（PNIPAAm）为温度敏感性材料的水凝胶，本文以两端带有碳碳双键的聚己内酯为交联剂，N-异丙基丙烯酰胺为单体，通过自由基共聚合成了一种生物可降解的温度敏感性水凝胶，并对其结构进行了表征，研究了此水凝胶体系在不同温度下的溶胀率，并通过对透光率的测试研究了水凝胶体系的温度响应性。另外，以此水凝胶体系作为载体研究了载有PTH1-34的水凝胶在不同温度下的释放行为。模拟释放结果表明，该水凝胶体系能实现所载多肽类药物的持续释放，可长达13天，因而在口腔种植领域有良好的应用前景。
[Abstract]:With the wide application of implant technology in clinic. Implant denture as a "third pair of human teeth" has become the first choice for more patients to repair missing teeth. The quality of alveolar bone and the volume of residual alveolar bone are the key factors to determine the initial stability of dental implants. However, more patients have the problem of insufficient bone mass around implants. The excellent osteogenesis of parathyroid hormone 1-34 (PTH1-34) has attracted wide attention of scholars. In the past, the experimental studies of PTH1-34 used to promote implant-bone healing were limited to systemic discontinuity and low dose administration, but PTH1-34 was characterized by variability and short half-life. It is very necessary to develop the carrier to control its slow release and to improve its bioavailability because of its high cost and so on. The environment-sensitive polymer hydrogel is one of the intelligent hydrogels. Can produce sensitive response to external stimuli. Because temperature is closely related to human physiological environment. Therefore, the research of temperature-sensitive hydrogels has been paid more and more attention. Temperature sensitive hydrogels can change their hydrophobicity and the size and volume of gel networks with the change of ambient temperature. Therefore, as a drug carrier, it can control the drug release rate with the change of temperature. At present, the most widely studied thermo-sensitive hydrogel is poly (N-isopropylacrylamide) PNIPAAm. A hydrogel that is a temperature-sensitive material. In this paper, a biodegradable thermo-sensitive hydrogel was prepared by free radical copolymerization of poly (caprolactone) with carbon-carbon double bond as crosslinking agent (N isopropyl acrylamide) as monomer. The structure of the hydrogel system was characterized, the swelling rate of the hydrogel system was studied at different temperatures, and the temperature response of the hydrogel system was studied by measuring the transmittance of the hydrogel system. The hydrogel system was used as the carrier to study the release behavior of the hydrogel containing PTH1-34 at different temperatures. The simulated release results showed that the hydrogel system could realize the sustained release of polypeptide drugs. It can last up to 13 days, so it has a good application prospect in the field of oral implant.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08


本文编号：1490780