低能量激光对人工骨成骨影响的体外研究

发布时间：2018-02-05 20:54

本文关键词： 低能量激光成骨样细胞人工骨血管内皮生长因子(VEGF)　出处：《吉林大学》2012年硕士论文　论文类型：学位论文

【摘要】：低能量激光（Low level laser，LLL）又称弱激光，其输出功率为毫瓦级。自Mester在激光免疫试验的基础上提出其具有生物刺激作用以来，众多学者对其作用机制及生物刺激效应等进行了深入的研究，将其应用于诊断、治疗等医疗领域，取得了优异的成果。经大量实验证明低能量激光具有调节免疫、促进组织修复及伤口愈合、减轻疼痛等作用。研究表明：低能量激光治疗能够促进成骨相关细胞的增殖、分化，促进骨基质的形成及细胞因子的分泌，促进骨组织愈合及修复，但将其应用于种植领域的研究较为少见，，尤其是对植骨后骨形成的影响。本实验尝试将低能量激光治疗应用于口腔种植领域，并验证不同照射剂量的LLL是否能有效促进成骨细胞增殖和是否能促进细胞与人工骨块的结合及对其血管化是否有影响。本研究采用了MTT方法检测人成骨样细胞MG-63的增殖；采用ELISA方法检测MG-63与人工骨块共培养体系经不同照射剂量的激光照射后，对血管内皮生长因子（VEGF）的分泌是否有促进作用，以此推断LLLT对人工骨植入后血管化的影响；扫描电镜观察细胞在人工骨块上的数目及结合情况。结果表明：适当照射剂量的低能量激光能够促进MG-63的增殖，能够促进MG-63与人工骨块共培养体系中成骨样细胞分泌VEGF因子，能够使细胞在骨块上的附着及生长状态更为良好。综上所述：低能量激光治疗能够促进人成骨样细胞的增殖、VEGF的分泌及其与人工骨块的结合，为LLLT应用于口腔种植临床提供了理论基础，对人工骨材料骨改建过程中血管化的促进提供了一种优质方法。
[Abstract]:Low level laser (LLL) is also called weak laser. The output power of Mester is milliwatt. Since Mester was proposed to have biological stimuli on the basis of laser immunoassay, many scholars have carried out in-depth research on the mechanism of its action and the effects of biological stimulation. It has been applied to diagnosis, treatment and other medical fields, and achieved excellent results. It has been proved by a large number of experiments that low energy laser can regulate immunity, promote tissue repair and wound healing. Low energy laser therapy can promote the proliferation and differentiation of osteoblast-associated cells, promote the formation of bone matrix and cytokine secretion, promote bone healing and repair. However, the study of its application in the field of implant is rare, especially the effect on bone formation after bone grafting. This experiment attempts to apply low-energy laser therapy to the field of oral implant. The results showed that different doses of LLL could effectively promote the proliferation of osteoblasts, promote the binding of the cells to the artificial bone graft and influence the vascularization of osteoblasts. In this study, MTT was used to detect the proliferation of human osteoblast-like cells (MG-63). ELISA method was used to detect whether MG-63 and artificial bone mass co-culture system could promote the secretion of vascular endothelial growth factor (VEGF) after different doses of laser irradiation. The effect of LLLT on vascularization after artificial bone implantation was inferred. Scanning electron microscopy (SEM) was used to observe the number and binding of cells on artificial bone mass. The results showed that low energy laser irradiation could promote the proliferation of MG-63. It can promote the secretion of VEGF factor by osteoblast like cells in the co-culture system of MG-63 and artificial bone graft, and make the cells attach and grow better on the bone mass. To sum up: low energy laser therapy can promote the proliferation of human osteoblast-like cells and the combination of LLLT with artificial bone mass, which provides a theoretical basis for the clinical application of LLLT in oral implants. It provides a high-quality method for promoting vascularization during bone remodeling of artificial bone.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.51

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