生物可吸收材料在关节骨折内固定中的生物相容性

发布时间：2018-02-10 05:16

本文关键词： 骨折固定术羟基磷灰石类组织工程生物材料材料相容性复合生物可吸收材料关节骨折内固定生物相容性生物可吸收性替代材料　出处：《中国组织工程研究》2016年34期 　论文类型：期刊论文

【摘要】：背景:近年来,聚L-乳酸/聚乳酸乙醇酸共聚物/羟基磷灰石复合生物可吸收材料在关节骨折中得到应用,效果理想。目的:分析聚L-乳酸/聚乳酸乙醇酸共聚物/羟基磷灰石复合生物可吸收材料在关节骨折内固定中的生物相容性。方法:(1)体内实验:取22只新西兰大兔,制备踝关节骨折模型,随机分2组,复合材料组采用聚L-乳酸复合生物可吸收材料固定,金属材料组采用金属支架固定。术后/聚乳酸乙醇酸共聚物/羟基磷灰石7 d,观察两组治疗效果及并发症情况;(2)体外实验:将聚L-乳酸/聚乳酸乙醇酸共聚物/羟基磷灰石复合生物可吸收材料与MG-63细胞共培养,观察细胞增殖、黏附。结果与结论:(1)体内实验结果:金属材料组术后9只兔骨折部位愈合良好,2只出现并发症;复合材料组骨折愈合良好,未出现并发症;(2)体外实验结果:接种于复合支架1 d后,细胞全部贴壁生长,多为梭形;培养3 d后细胞数量开始增加,排列紧密且相对规则,显示出旺盛的生长能力,培养1-7 d的细胞毒性为0或1级。共培养3 d时,复合材料表面细胞为梭形或多边形,细胞伸展良好,细胞之间存在明显的连接,并且细胞表面的毛躁部分深入材料表面进行生长,表现出良好的黏附性;(3)结果表明:聚L-乳酸/聚乳酸乙醇酸共聚物/羟基磷灰石复合生物可吸收材料具有良好的生物相容性。
[Abstract]:Background: in recent years, poly (L-lactic acid) / poly (lactic acid glycolate) / hydroxyapatite composite bioabsorbable materials have been applied in joint fractures. Objective: to analyze the biocompatibility of poly (L-lactic acid) / poly (lactic acid glycolate) / hydroxyapatite composite bioabsorbable material in the internal fixation of joint fracture. The ankle fracture model was established and randomly divided into two groups. The composite group was fixed with Poly (L-lactic acid) composite bioabsorbable material. The metal material group was fixed with metal scaffold. After operation / poly (lactic acid glycolate copolymer / hydroxyapatite) for 7 days, the therapeutic effect and complications of the two groups were observed in vitro: Poly (lactic acid / polylactic acid glycolate copolymer / poly (lactic acid) / poly (lactic acid glycolate) /. Hydroxyapatite composite bioabsorbable materials co-cultured with MG-63 cells. Observation of cell proliferation and adhesion. Results and conclusions in vivo experiment results: 9 rabbits in the metal material group had good fracture healing and 2 had complications, while the composite group had good fracture healing. Results of in vitro experiment: after inoculation with the composite scaffold for 1 day, all the cells adhered to the wall, most of them were fusiform, and after 3 days of culture, the number of cells began to increase, and the arrangement was tight and regular, showing strong growth ability. When cultured for 3 days, the cells on the composite surface were fusiform or polygonal, the cells extended well, and there were obvious connections between the cells. The results showed that poly (lactic acid) / poly (lactic acid glycolate copolymer / hydroxyapatite) composite bioabsorbable materials had good biocompatibility.
【作者单位】：贵州省六盘水市水矿控股集团总医院骨一科;遵义医学院附属医院骨科;
【分类号】：R318.08;R683

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