纯钛超声工作尖与传统工作尖对钛表面形态及人牙周膜细胞黏附强度影响的比较研究

发布时间：2018-02-12 03:50

  本文关键词： 纯钛超声工作尖 传统材料超声工作尖 人牙周膜细胞 粗糙度 粘附强度　出处：《南京大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的比较经纯钛超声工作尖和传统工作尖刮治后的钛表面形态变化以及对人牙周膜细胞(Humen periodontal ligament cells,hPDLCs)黏附强度的影响。方法利用纯钛超声工作尖和传统工作尖分别对60个光滑钛片进行刮治,刮治时间为10s和30s。随后将其分为五组:光滑钛片对照组(C,12个),纯钛超声工作尖刮治1Os组(Ti10,12个),纯钛超声工作尖刮治刮治30s组(Ti30,12个),传统工作尖刮治10s组(Cv10,12个),传统工作尖刮治30s组(Cv30,12个)。刮治后使用扫描电镜(Scanning electron microscope,SEM)观察钛片表面的形态变化,并用表面粗糙度轮廓仪测量钛片表面的粗糙度;将hPDLCs与各组钛片共培养12h后,利用CCK8(Cell Counting Kit 8)法检测细胞的黏附强度。结果SEM和表面粗糙度轮廓仪显示,各组钛片粗糙度与对照组相比都显著增加(P0.05);Ti10组钛片表面的粗糙度明显小于Cv10组(P0.05);Ti30组与Cv30组间的钛片表面粗糙度无显著差异(P0.05);同种材料,不同处理时间未造成显著差异(P0.05);两种工作尖处理后,不同粗糙度的钛表面对hPDLC的黏附强度无统计学差异(P0.05)。结论在短时间内(10s)纯钛超声工作尖对钛表面形态及粗糙度的改变较传统工作尖更小,但是延长工作时间后(30s),该差异消失。经两种工作尖处理后的钛片表面对人牙周膜细胞的黏附强度差异无显著性。
[Abstract]:Objective to compare the changes of titanium surface morphology and its effect on the adhesion strength of human periodontal periodontal ligament cells hPDLCsafter curettage of pure titanium working point and traditional working point. Methods the effects of pure titanium ultrasonic working point and traditional working tip on the adhesion strength of human periodontal ligament cells were compared. 60 smooth titanium slices were curetted, The curettage time was 10 s and 30 s. Then it was divided into five groups: smooth titanium slice control group (C, n = 12), pure titanium ultrasonic working tip curettage group (n = 12), pure titanium ultrasonic working tip curettage group (n = 30), curettage group (n = 12), conventional working tip group (n = 10), Cv10 group (n = 12), and ultrasonic curettage group (n = 12). After curettage, scanning electron microscopes (SEM) were used to observe the morphological changes of titanium surface. The surface roughness of titanium sheet was measured by surface roughness profilometer, and the adhesion strength of the cells was measured by CCK8(Cell Counting Kit 8 method after co-culture of hPDLCs and each group of titanium slices for 12 hours. Results SEM and surface roughness profilometer showed that, Compared with the control group, the surface roughness of each group was significantly higher than that of the control group. The surface roughness of the Ti10 group was significantly lower than that of the Cv10 group. There was no significant difference in the surface roughness between the Cv30 group and the Cv30 group. There was no significant difference between different treatment time (P 0.05). There was no significant difference in adhesion strength of titanium surfaces with different roughness to hPDLC. Conclusion the change of surface morphology and roughness of pure titanium working tip is smaller than that of traditional working tip in a short time. However, the difference disappeared after prolonging the working time for 30 s. There was no significant difference in the adhesion strength of the titanium surface treated with two working tips to human periodontal ligament cells.
【学位授予单位】：南京大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R783.1
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本文编号：1504719