镓掺杂β-磷酸三钙骨修复材料的研究

发布时间：2018-02-21 08:06

本文关键词： 骨组织工程 β-磷酸三钙镓生物相容性细胞毒性　出处：《长江大学》2017年硕士论文　论文类型：学位论文

【摘要】：骨骼是人体内最坚硬的器官,对人们正常的生活有重要作用,一旦出现骨损伤,会对人体会造成巨大的伤害。近年来,骨损伤患者越来越多,临床上目前常用的治疗方法是进行骨修复材料填充。无机生物陶瓷材料中的β-磷酸三钙的组成与人体内骨的无机盐成分相似,在人体内有良好的生物相容性及生物降解性,降解后产生的钙、磷等元素可被人体吸收用于骨的重建,是目前骨修复领域研究的重点方向之一。为了更进一步提高β-磷酸三钙在骨组织修复过程的性能,可以通过离子掺杂法来改变其理化性质和生物学性能。人体中很多微量元素具有重要的生化作用。镓作为人体内非必需微量元素,能够抑制破骨细胞再吸收,抑制骨溶解,阻止骨钙释放,改变骨中Ⅰ型胶原和纤维蛋白的基因表达,有利于新骨的形成,还能增加骨骼中钙和磷的含量,直接作用于人体骨的形成。因此本课题提出把镓掺杂进β-磷酸三钙中,进一步改善和提高其生物活性,并系统研究其相关性能的变化。本试验采用湿法工艺法按(Ca+Ga)/P=1.5的比例合成0%、5%、10%镓掺杂β-磷酸三钙粉体。XRD分析表明,镓掺杂β-磷酸三钙多孔骨修复材料保持纯净且结晶度较好。镓的引入导致β-磷酸三钙晶格收缩,镓掺杂量越大,晶体体积收缩越明显。傅里叶红外光谱分析表明,随着镓离子掺入量的增加,β-磷酸三钙的特征峰强度逐渐减弱。电镜形貌分析表明,镓掺杂β-磷酸三钙多孔骨修复材料内部有三维网状结构的大孔与贯通性良好的微孔结构,有利于骨组织的修复,符合骨替代材料的要求。在制备镓掺杂β-磷酸三钙骨修复材料时,选择浓度为5 wt%的聚乙烯醇溶液作为粘结剂,25 wt%硬脂酸为致孔剂,可使得镓掺杂β-磷酸三钙多孔骨修复材料在机械强度和孔隙率之间达到最优,并且无杂质引入。在模拟体液中对镓掺杂β-磷酸三钙骨修复材料进行降解试验,经X射线衍射及傅里叶红外分析可知掺镓β-磷酸三钙骨修复材料表面有类骨磷灰石物质沉积,随着镓掺杂量增加,新生成物质的结晶度有一定增强。利用MTT法进行细胞增殖试验,结果表明掺镓β-磷酸三钙骨修复材料无细胞毒性,对于细胞的增殖有一定促进作用。碱性磷酸酶测定表明试验掺镓β-磷酸三钙骨修复材料能促进成骨细胞中ALP的分泌。根据一系列试验结果可知,掺镓β-磷酸三钙骨修复材料有较好的可降解性及生物相容性,是一种有前景的骨修复材料。
[Abstract]:Bone is the hardest organ in the human body and plays an important role in people's normal life. Once bone injury occurs, it will cause great harm to human body. In recent years, more and more patients with bone injury are suffering from bone injury. At present, the commonly used treatment method is bone repair material filling. The composition of 尾 -tricalcium phosphate in inorganic bioceramics is similar to the inorganic salt composition of human bone, and has good biocompatibility and biodegradability in human body. Calcium, phosphorus and other elements produced by degradation can be absorbed by the human body for bone reconstruction, which is one of the important research directions in the field of bone repair. In order to further improve the performance of 尾 -tricalcium phosphate in bone tissue repair, The physical and chemical properties and biological properties can be changed by ion doping. Many trace elements in the human body have important biochemical effects. Gallium, as a non-essential trace element in human body, can inhibit the reabsorption of osteoclasts and inhibit osteolysis. Blocking the release of bone calcium, altering the gene expression of type I collagen and fibrin in bone, which is beneficial to the formation of new bone, can also increase the contents of calcium and phosphorus in bone. Therefore, this paper proposes that doping gallium into 尾 -tricalcium phosphate can further improve and enhance its biological activity. In this experiment, the method of wet method was used to synthesize 0% 10% gallium doped 尾 -tricalcium phosphate powder according to the ratio of Ca Ga)/P=1.5, and the results showed that the content of Ga-doped 尾 -tricalcium phosphate powder was better than that of 尾 -tricalcium phosphate powder. Gallium doped 尾 -tricalcium phosphate porous bone repair materials keep pure and good crystallinity. The introduction of gallium leads to the contraction of 尾 -tricalcium phosphate lattice, the larger the doping amount of gallium, the more obvious the shrinkage of crystal volume. The characteristic peak intensity of 尾 -tricalcium phosphate gradually decreased with the increase of gallium ion incorporation. Electron microscope analysis showed that there were macropores with three-dimensional reticular structure and micropore structure with good transfixibility in Gallium doped 尾 -tricalcium phosphate porous bone repair materials. When preparing Gallium doped 尾 -tricalcium phosphate bone repair material, polyvinyl alcohol solution with concentration of 5 wt% was selected as binder for 25 wt% stearic acid as pore-forming agent. Gallium doped 尾 -tricalcium phosphate porous bone repair material is optimized between mechanical strength and porosity, and no impurity is introduced. The degradation test of gallium doped 尾 -tricalcium phosphate bone repair material is carried out in simulated body fluid. X-ray diffraction and Fourier transform infrared analysis showed that bone-like apatite was deposited on the surface of Gallium-doped 尾 -tricalcium phosphate bone repair material, and with the increase of doping amount of gallium, The crystallinity of the newly formed material was enhanced to some extent. Cell proliferation test was carried out by MTT method. The results showed that there was no cytotoxicity in the Gallium 尾 -tricalcium phosphate repair material. Alkaline phosphatase assay showed that Gallium 尾 -tricalcium phosphate could promote the secretion of ALP in osteoblasts. Gallium 尾 -tricalcium phosphate is a promising bone repair material with good biocompatibility and biodegradability.
【学位授予单位】：长江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08

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