中空纤维透析膜制备、传递特性及生物相容性

发布时间：2018-02-22 23:48

  本文关键词： 组织工程 生物相容性材料 血液透析液 生物材料 材料相容性 血液透析 一缩二乙二醇 聚乙烯吡咯烷酮 N N-二甲基乙酰胺 非溶剂致相分离法 中空纤维透析膜 Ⅱ型医用聚氨酯材料 血液相容性　出处：《中国组织工程研究》2017年02期 　论文类型：期刊论文

【摘要】：背景:聚砜膜具备良好的抗生物抗降解性能,但临床上对于以聚砜作为材料制备中空纤维透析膜的制备方法及血液相容性缺乏统一的标准。目的:观察中空纤维透析膜的制备方法、传递特性及材料生物相容性。方法:以聚砜作为膜材料,将一缩二乙二醇作为致孔剂,聚乙烯吡咯烷酮作为改性剂,N,N-二甲基乙酰胺作为溶剂,利用非溶剂致相分离法制备中空纤维透析膜。采用扫描电镜、超景深三维显微镜成像及孔隙率测试等测定透析膜的性能;利用超滤装置完成传递参数截留率和水通量参数测定;通过溶血实验、动态凝血时间实验及血小板黏附实验测定中空纤维透析膜的血液相容性,以Ⅱ型医用聚氨酯材料作为阴性对照。结果和结论:1中空纤维透析膜形态:制备的中空纤维透析膜断面不对称,质量分数17%透析膜中间层呈大孔状结构,而质量分数19%,21%及23%的透析膜制备的中空纤维透析膜呈海绵状结构;2中空纤维透析膜孔隙率:中空纤维透析膜和Ⅱ型医用聚氨酯材料在膜面积相同的情况下,中空纤维透析膜密度显著低于,孔隙率显著大于阴性对照材料(P0.05);3中空纤维透析膜传递性能:中空纤维透析膜水体积及水通量,显著高于阴性对照材料(P0.05);4中空纤维透析膜血液相容性:两种材料共进行3次溶血实验结果显示:中空纤维透析膜平均吸光度值和溶血率显著高于阴性对照材料(P0.05);动态凝血时间实验及血小板黏附实验结果显示,中空纤维透析膜20,40,70 min动态凝血时间显著短于阴性对照材料(P0.05)。结果提示:将聚砜作为膜材料,利用非溶剂致相分离法能程控制备生物相容性较好的中空纤维透析膜,材料具有良好的血液相容性及传递效率。
[Abstract]:Background: polysulfone membrane has good anti-biodegradability. But there is no uniform standard for the preparation and blood compatibility of hollow fiber dialysate membrane using polysulfone as the material in clinic. Objective: to observe the preparation method of hollow fiber dialysis membrane. Methods: Poly (sulfone) was used as membrane material, diethylene glycol was used as pore-forming agent, polyvinylpyrrolidone as modifier, N-dimethyl acetamide as solvent, polysulfone as membrane material, and polyvinylpyrrolidone as modifier. Hollow fiber dialysis membrane was prepared by non-solvent phase separation method. The properties of dialysis membrane were measured by scanning electron microscope (SEM), 3D imaging of depth of field and porosity test, and the parameters of transfer parameter rejection and water flux were measured by ultrafiltration device. The blood compatibility of hollow fiber dialysis membrane was determined by hemolysis test, dynamic coagulation time test and platelet adhesion test. Results and conclusion: the morphology of the hollow fiber dialysis membrane: the section of the hollow fiber dialysis membrane prepared was asymmetric, and the intermediate layer of 17% mass fraction dialysis membrane was macroporous. However, the hollow fiber dialysate membrane prepared with 21% and 23% mass fraction of hemodialysis membrane showed spongy structure and hollow fiber dialysate membrane porosity: under the same membrane area, the hollow fiber dialysis membrane and the type 鈪，

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