γ显像在层析分离及蛋白分子位点密度测定中的应用

发布时间：2018-02-23 16:14

本文关键词： 体层摄影术发射型计算机单光子层析分离 E-选择素位点密度　出处：《生物医学工程学杂志》2017年03期 　论文类型：期刊论文

【摘要】：本文用放射性核素标记以及单光子发射型计算机断层(SPECT)γ显像的方法,进行E-选择素蛋白分子位点密度测定,以探讨其实用价值。采用Indogen法对E-选择素蛋白分子抗体进行125I标记,标记产物经G25葡聚糖凝胶层析柱分离纯化,对分离出的样品排列在塑胶试管架上,用SPECT进行平面显像并定量分析。制备不同浓度的E-选择素标准溶液,将放射性计数换算为位点密度,作蛋白量位点密度标准曲线。标记产物经SPECT定量分析,E-选择素抗体标记率为78%;不同浓度样品饱和曲线显示,当浓度在0~1 mg/m L浓度范围时,得标准曲线为y=6 045.7x—51.166,决定系数R~2=0.997 9。研究结果表明,γ显像在放射性标记产物分析及位点密度测定时有一定实用价值。
[Abstract]:The method of radionuclide labeling and single photon emission computed tomography (SPECT) 纬 imaging was used to determine the molecular site density of Eselectin protein in order to explore its practical value. The molecular antibody of Eselectin protein was labeled with 125i by Indogen method. The labeled products were separated and purified by G25 dextran gel chromatography column. The samples were arranged on the plastic tube stand. The standard solution of E- selectin was prepared by using SPECT for planar imaging and quantitative analysis. The radioactivity count was converted to the site density and used as the standard curve of protein quantity site density. The labeled product was quantitatively analyzed by SPECT and the labeling rate of Eselectin antibody was 78. The saturation curve of different concentration samples showed that when the concentration was in the concentration range of 0 ~ 1 mg/m / L, The standard curve is yang6045.7x-51.166, and the determinant coefficient is R ~ (2 +) (0.997 9). The results show that 纬 imaging has some practical value in the analysis of radioactive labeling products and the determination of site density.
【作者单位】：暨南大学附属第一医院核医学科;广州军区广州总医院核医学科;华南理工大学生物科学与工程学院;
【基金】：广东省自然科学基金(2016A030313612)
【分类号】：R817

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