脊髓损伤的PLGA低温成形组织工程支架修复

发布时间：2018-02-26 16:05

本文关键词： 组织工程低温沉积制造支架脊髓损伤雪旺细胞　出处：《中国生物医学工程学报》2017年01期 　论文类型：期刊论文

【摘要】：组织工程的迅速兴起给脊髓损伤后的神经功能恢复带来了新的希望,而三维结构组织工程支架成为用组织工程方法治疗脊髓损伤的关键因素。尝试采用PLGA低温成形支架修复脊髓损伤。采用PLGA作为生物支架材料,通过低温快速成形工艺制备脊髓支架,采用雪旺细胞进行细胞培养实验,选用30只SD大鼠分组进行支架修复脊髓损伤的动物实验,并对实验结果进行组织切片和染色表征。对支架的各项性能进行表征后,结果表明支架的平均孔隙率达到87.64%,具有良好的亲水性。支架降解速率表现出前期慢、后期加快的趋势,实验中支架在前16周表现良好的稳定性,从20周起逐步加快。各项性能能满足组织工程脊髓支架的要求。观察显示,雪旺细胞在PLGA支架上生长、增殖情况良好,支架细胞相容性良好,适合细胞黏附和生长。BBB评分显示,实验组大鼠截瘫情况从第4周起有好转迹象,受伤大鼠后肢观察到轻微活动迹象。不同切片染色观察结果表明,该组织工程支架对于脊髓损伤修复均有不同程度的促进,在阻碍胶质癜痕生成、促进神经纤维及髓鞘愈合方面取得不同程度的效果。观察表明,该方法能够在脊髓损伤修复中取得实质性的治疗效果。
[Abstract]:The rapid rise of tissue engineering brings new hope to the recovery of neural function after spinal cord injury. Three-dimensional scaffold for tissue engineering has become a key factor in the treatment of spinal cord injury. The PLGA cryogenic scaffold was used to repair spinal cord injury. PLGA was used as biomaterial for spinal cord injury. Spinal cord scaffolds were prepared by low temperature rapid prototyping. Schwann cells were used for cell culture and 30 SD rats were divided into two groups. The results showed that the average porosity of the scaffold was 87.64, and the scaffold had good hydrophilicity. The degradation rate of the scaffold was slow in the early stage and accelerated in the later stage. The stents showed good stability in the first 16 weeks and accelerated gradually from the 20th week. The performance of the scaffolds could meet the requirements of tissue engineering spinal cord scaffolds. The observation showed that Schwann cells grew well on the PLGA scaffold, and the proliferation of Schwann cells was good. The scaffold had good cytocompatibility, suitable for cell adhesion and growth. BBB score showed that the paraplegia in the experimental group showed signs of improvement from the 4th week, and slight signs of movement were observed in the injured hind limbs. The tissue engineering scaffold can promote the repair of spinal cord injury in different degree, and has different effects on blocking the formation of glial scar and promoting the healing of nerve fiber and myelin sheath. This method can achieve substantial therapeutic effect in spinal cord injury repair.
【作者单位】：杭州电子科技大学生物制造研究中心;
【基金】：国家自然科学基金(61272389,60952001)
【分类号】：R318.08;R651.2

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