骨形态发生蛋白、碱性成纤维细胞生长因子生物材料在关节软骨缺损修复中的生物性能

发布时间：2018-02-28 18:16

本文关键词：关节软骨骨形态发生蛋白质类成纤维细胞生长因子组织构建软骨组织工程骨形态发生蛋白碱性成纤维细胞生长因子生物材料关节软骨缺损生物性能膝关节软骨细胞修复材料　出处：《中国组织工程研究》2016年20期 　论文类型：期刊论文

【摘要】：背景:众多的实验中发现了多种细胞因子通过自分泌和旁分泌等不同方式调节软骨和骨的生成。目的:分析骨形态发生蛋白、碱性成纤维细胞生长因子生物材料在关节软骨缺损修复中的生物性能。方法:选取40只新西兰家兔,随机分为4组,纤维蛋白组、碱性成纤维细胞生长因子组、骨形态发生蛋白组、复合组(骨形态发生蛋白+碱性成纤维细胞生长因子),每组10只。建立兔关节软骨缺损模型,止血彻底后将纤维蛋白、碱性成纤维细胞生长因子、骨形态发生蛋白以及骨形态发生蛋白、碱性成纤维细胞生长因子复合等材料组成的支架分别植入缺损部位。比较不同注射材料在家兔关节软骨缺损中的效果及复合材料的生物性能。结果与结论:(1)关节软骨缺损修复情况:纤维蛋白组2只家兔出现跛行;碱性成纤维细胞生长因子组1只家兔活动受限;骨形态发生蛋白组出现1只跛行,1只活动受限;复合组兔恢复良好,膝关节和手术前相比差异无显著性意义(P0.05)。(2)大体观察:复合组家兔软解软骨缺损消失,内有新生血管,软骨和正常组织十分接近;骨形态发生蛋白组关节软骨边际存在裂隙,未能与正常的软骨组织紧密结合,光镜下能够看见缺损区周缘存在软骨细胞;纤维蛋白组缺损部位和周围组织基本愈合;碱性成纤维细胞生长因子组缺损部位有所修复,但不光滑。(3)苏木精-伊红染色结果:纤维蛋白组兔缺损部位未被修复,表面存在明显凹陷;碱性成纤维细胞生长因子组缺损部位被明显修复,缺损部位存在大量软骨细胞;骨形态发生蛋白组被修复,出现软骨细胞,但排列不规则;复合组修复良好,出现大量软骨细胞。(4)结果提示,骨形态发生蛋白、碱性成纤维细胞生长因子生物材料是关节软骨缺损中比较理想的修复材料,能够发挥骨形态发生蛋白诱导形成软骨的活性,能够发挥碱性成纤维细胞生长因子提高软骨细胞增生作用,可以达到优势互补,促进关节软骨缺损的恢复。
[Abstract]:Background: in many experiments, many cytokines have been found to regulate cartilage and bone formation through autocrine and paracrine, etc. Objective: to analyze bone morphogenetic protein (BMP). Biological properties of basic fibroblast growth factor biomaterials in repair of articular cartilage defect methods: 40 New Zealand rabbits were randomly divided into 4 groups: fibrin group, basic fibroblast growth factor group, and fibroblast growth factor group. Bone morphogenetic protein group, composite group (bone morphogenetic protein basic fibroblast growth factor, 10 in each group. Rabbit articular cartilage defect model, after hemostasis thoroughly fibrin, basic fibroblast growth factor, Bone morphogenetic proteins and bone morphogenetic proteins, The scaffolds composed of basic fibroblast growth factor (bFGF) and other materials were implanted into the defect site respectively. The effects of different injection materials on articular cartilage defects in rabbits and the biological properties of composite materials were compared. Repair of cartilage defect: lameness appeared in 2 rabbits in fibrin group; In the basic fibroblast growth factor group, the activity of one rabbit was restricted, the bone morphogenetic protein group showed a limping limitation, and the compound group recovered well. There was no significant difference between the knee joint and the operation before operation (P 0.05). Gross observation: in the composite group, the defect of soft decomposed cartilage disappeared, there were neovascularization in the cartilage, the cartilage and normal tissue were close to each other, and the bone morphogenetic protein group had a crack in the articular cartilage margin. The defect site and surrounding tissue were basically healed in fibrin group, and the defect site was repaired in basic fibroblast growth factor group. However, the results of hematoxylin-eosin staining showed that the defect site was not repaired in fibrin group, and there was a significant depression on the surface of the defect, and a large number of chondrocytes were found in the defect site in basic fibroblast growth factor group. Bone morphogenetic protein group was repaired with irregular arrangement of chondrocytes, and the composite group was well repaired with a large number of chondrocytes. Basic fibroblast growth factor (bFGF) biomaterials are ideal repair materials for articular cartilage defects, which can play the role of bone morphogenetic protein-induced cartilage formation. It can play the role of basic fibroblast growth factor to enhance the proliferation of chondrocytes, to achieve complementary advantages, and to promote the recovery of articular cartilage defects.
【作者单位】：南阳医学高等专科学校第一附属医院;郑州大学第一附属医院;
【基金】：郑州市科技局科研项目资助(20150138)~~
【分类号】：R318.08;R684

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