应用微纤维胶原支架构建组织工程软骨

发布时间：2018-03-01 03:30

  本文关键词： 纤维胶原类 软骨细胞 软骨 组织工程 生物材料 软骨生物材料 微纤维胶原 国家自然科学基金　出处：《中国组织工程研究》2017年22期 　论文类型：期刊论文

【摘要】：背景:寻找理想的组织工程支架材料,仍然是软骨组织工程学的重要课题之一。目的:探讨微纤维胶原应用于软骨组织工程支架的可行性。方法:手术切取兔耳软骨,提取原代软骨细胞,并进行扩增培养。将海绵状微纤维胶原用无菌刀片切割成小方块,取第2代软骨细胞混悬液接种于微纤维胶原支架材料上。体外培养1周后,植入裸鼠体内,观察8周后取材,进行大体、组织学、免疫组织化学等方法观察。结果与结论:(1)在细胞接种过程中,湿水之后的微纤维胶原支架无明显缩水,仍然能够保持其立体形态;(2)体外培养1周以及植入体内8周后,无明显体积和形态变化;(3)裸鼠体内培养8周后形成白色半透明且富有弹性的成熟软骨块;(4)组织学观察可见所构建的软骨组织成熟,基质分泌旺盛,组织内仍可见未完全降解的微纤维胶原材料;(5)结果表明,微纤维胶原蛋白是一种良好的软骨组织工程支架材料。
[Abstract]:Background: searching for ideal scaffold materials for tissue engineering is still one of the important topics in cartilage tissue engineering. Objective: to explore the feasibility of the application of microfiber collagen in cartilage tissue engineering scaffolds. The primary chondrocytes were extracted and cultured. The spongy microfibrous collagen was cut into small cubes with aseptic blades, and the second passage chondrocytes were mixed with the scaffold of microfiber collagen. After 1 week of culture in vitro, the chondrocytes were cultured for 1 week. Results and conclusion: during cell inoculation, microfiber collagen scaffolds did not shrink significantly after wetting water, and the microfibrillar collagen scaffolds did not shrink after wetting water, and the microfibrillar collagen scaffolds were taken from nude mice after 8 weeks of observation, and were observed by gross, histological and immunohistochemical methods. Still able to maintain its stereomorphology for 1 week in vitro and 8 weeks after implantation. After 8 weeks of culture in nude mice, white translucent and elastic mature chondrocytes were formed. Histological observation showed that the chondrocytes were mature and exuberant in matrix secretion. The results showed that microfibrillar collagen was a good scaffold for cartilage tissue engineering.
【作者单位】： 广州医科大学附属口腔医院 广州口腔疾病研究所 口腔医学重点实验室;解放军第306医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金青年科学基金项目(81401609)~~
【分类号】：R318.08
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