静压力对脂肪干细胞与静电纺丝纳米纤维细胞相容性的影响

发布时间：2018-03-01 09:55

本文关键词： 脂肪组织干细胞纳米纤维压力组织工程生物材料纳米材料静压力脂肪干细胞静电纺丝增殖黏附细胞活性细胞相容性国家自然科学基金　出处：《中国组织工程研究》2016年30期 　论文类型：期刊论文

【摘要】：背景:静电纺丝聚乳酸/聚己内酯纳米纤维是课题组自行合成的生物可降解材料,前期研究表明该材料生物相容性优越,但缺乏对该材料力学方面的研究。目的:研究静压力对脂肪干细胞与静电纺丝聚乳酸/聚己内酯纳米纤维支架细胞相容性的影响。方法:将脂肪干细胞接种到由聚乳酸和聚己内酯合成的静电纺丝纳米纤维支架上,置于含体积分数为10%胎牛血清的DMEM低糖培养基中培养。利用静压力装置分别对培养板持续加载0,15,30,45 k Pa的静压力,加载时间为4 h。采用MTT法、活死细胞染色检测脂肪干细胞在静电纺丝纳米纤维支架上的增殖、黏附和活性,评价脂肪干细胞与静电纺丝纳米纤维的细胞相容性。结果与结论:(1)在体外培养条件下,经不同静压力持续加载4 h后,MTT法增殖检测得出的各组吸光度值经单因素方差分析差异存在显著性意义。在0-30 k Pa压力范围内,吸光度值随静压力的增加而增加,到45 k Pa时,吸光度值反而下降。各组间多重比较显示差异均有显著性意义;(2)用MTT法进一步检测细胞黏附百分率,各组间差异亦有显著性意义;(3)活死细胞染色结果印证了上述结果,统计分析表明,无论是单因素方差分析还是各组间配对t检验,4组间活细胞百分率的差异均有非常显著性意义;(4)适当大小的静压力可促进脂肪干细胞与静电纺丝纳米纤维的生物相容性,细胞增殖、黏附及活性有所提高,但过高的静压力会抑制细胞的生物学行为,从而影响脂肪干细胞与静电纺丝纳米纤维的相容性。
[Abstract]:Background: Electrospun poly (lactic acid) / poly (caprolactone) nanofibers are biodegradable materials synthesized by our team. Objective: to study the effect of static pressure on the compatibility of adipose stem cells with electrostatic spinning poly (lactic acid) / poly (caprolactone) nanofiber scaffolds. On the scaffold of electrospun nanofibers synthesized by polylactic acid and polycaprolactone, DMEM medium containing volume fraction of 10% fetal bovine serum was cultured. The culture plate was continuously loaded with static pressure of 0 1530U 45 KPA by static pressure device for 4 h. MTT method was used. The proliferation, adhesion and activity of adipose stem cells on electrostatic spinning nanofilament scaffolds were detected by living cell staining. The cytocompatibility of adipose stem cells with electrostatic spinning nanofibers was evaluated. After 4 hours of continuous loading under different static pressure, the absorbance values of each group were significantly different by single factor ANOVA. In the range of 0-30 KPA pressure, the absorbance value increased with the increase of static pressure, and reached 45 KPA. The absorbance value decreased instead. The multiple comparisons among the groups showed that there were significant differences between the two groups. The cell adhesion percentage was further detected by MTT method. There was also a significant difference between the three groups. The results of the staining of living dead cells confirmed the above results. Statistical analysis shows that. There were significant differences in the percentage of living cells between the four groups in both univariate ANOVA and paired t test. (4) the biocompatibility of adipose stem cells with electrostatic spinning nanofibers was promoted by static pressure. Cell proliferation, adhesion and activity were increased, but high static pressure inhibited the biological behavior of the cells, which affected the compatibility of adipose stem cells with electrostatic spinning nanofibers.
【作者单位】：华中科技大学同济医学院附属同济医院泌尿外科;武汉大学口腔医学院口腔医院种植科;武汉理工大学材料科学与工程学院;武汉市中心医院泌尿外科;
【基金】：国家自然科学基金青年基金(31400837) 华中科技大学自主创新基金(2013),华中科技大学同济医学院附属同济医院科学研究基金(2012)~~
【分类号】：R318.08

【相似文献】