肽核酸芯片原位合成系统设计

发布时间：2018-03-05 15:28

本文选题：肽核酸芯片　切入点：光导向法　出处：《中南大学》2012年硕士论文　论文类型：学位论文

【摘要】：基因芯片技术日臻完善,但芯片探针的特异性与灵敏度之间的矛盾仍然是基因芯片在分子生物学和生物医学方面应用研究的突出问题。使用肽核酸(PNA)做为芯片探针有望解决上述的问题。本文致力于基于光导向法的PNA芯片原位合成系统的设计与实现,旨在开发用于合成中、高密度PNA芯片的自动化设备。本文重点阐述了PNA芯片原位合成系统的设计与组装,系统包括：虚拟掩模光刻系统、自动加样系统及反应池装置。研究内容包括：在原有的虚拟掩模光刻系统的基础上开发曝光控制软件,通过控制数字微镜器件(DMD)的图像输出,实现空间准确定位的多重光脱保护反应；设计以可编程逻辑控制器(PLC)为控制核心的自动加样系统,通过气源动力装置、预活化装置及多向流体阀装置,为固相PNA合成过程提供试剂的输送、混合及洗涤等操作。本PNA原位合成系统在软件指令的控制下,通过循序若干次的光脱保护反应、单体预活化处理、偶联反应及盖帽反应,实现基于光导向法的PNA芯片的原位合成。本合成系统基本满足中、高密度PNA芯片的合成需求,为PNA芯片的推广及应用打下基础。
[Abstract]:Gene chip technology is improving, However, the contradiction between the specificity and sensitivity of microarray probes is still a prominent problem in the application of gene chips in molecular biology and biomedicine. The use of peptide nucleic acid (PNA) as chip probes is expected to solve the above problems. This paper is devoted to the design and implementation of PNA chip in situ synthesis system based on optical guidance method. Designed to develop automation equipment for synthetic, high-density PNA chips. This paper focuses on the design and assembly of PNA chip in situ synthesis system. The system includes: virtual mask lithography system. The research contents include: developing exposure control software on the basis of the original virtual mask lithography system, and controlling the image output of the digital micromirror device (DMD). The automatic sampling system with PLC as the control core is designed, and the automatic sampling system is designed through the pneumatic power device, preactivation device and multidirectional fluid valve device. This PNA in-situ synthesis system, controlled by software instruction, is controlled by several successive optical deprotection reactions, monomer preactivation, coupling reactions and capping reactions. In-situ synthesis of PNA chip based on optical guidance method is realized. This synthesis system basically meets the synthesis demand of high density PNA chip, which lays a foundation for the popularization and application of PNA chip.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.6

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