同种异体脂肪干细胞复合纳米级胶原基骨材料修复尺骨缺损

发布时间：2018-03-11 02:37

本文选题：干细胞　切入点：脂肪组织　出处：《中国组织工程研究》2015年32期 　论文类型：期刊论文

【摘要】：背景:随着医学模式的不断发展进步,人们对于骨缺损的治疗以及后期康复水平提出了更高的要求,这就使得以干细胞培养为基础的骨组织工程技术成为骨缺损治疗中较为重要的组成部分。目的:探讨同种异体脂肪干细胞复合纳米级胶原基骨材料修复兔尺骨缺损的可行性。方法:选取成年雌性新西兰兔70只作为研究对象,其中10只用于脂肪间充质干细胞的分离培养,60只采取随机数字表法分为单纯支架组、支架材料结合自体骨组、支架材料结合异体脂肪干细胞组,每组20只,均行尺骨中段部分切除,构建12 mm的骨缺损,然后植入相应的材料。植入材料6,9,12周测量骨密度,植入材料14周后观察3组坚固融合、非坚固融合、假关节形成比例。结果与结论:支架材料结合异体脂肪干细胞组坚固融合比例显著高于单纯支架组、支架材料结合自体骨组,非坚固融合及假关节比例显著低于单纯支架组、支架材料加自体骨组(P0.05)。单纯支架组坚固融合比例显著低于支架材料结合自体骨组,非坚固融合及假关节比例显著高于支架材料结合自体骨组(P0.05)。支架材料结合异体脂肪干细胞组植入材料后第6,9,12周骨密度明显高于单纯支架组、支架材料结合自体骨组(P0.05)。单纯支架组骨密度明显低于支架材料结合自体骨组(P0.05)。结果表明同种异体脂肪干细胞复合纳米级胶原基骨材料修复尺骨缺损效果确切,骨密度较高。
[Abstract]:Background: with the continuous development and progress of medical model, people put forward higher requirements for the treatment of bone defects and the level of rehabilitation in the later stage. This makes bone tissue engineering based on stem cell culture an important part in the treatment of bone defect. Objective: to explore the repair of rabbit ulnar defect with allogeneic adipose stem cells combined with nano-collagen bone materials. Methods: 70 adult female New Zealand rabbits were selected as subjects. Among them, 10 of them were isolated and cultured for adipose mesenchymal stem cells. 60 of them were randomly divided into two groups: scaffold group, scaffold material combined with autologous bone group, scaffold material combined with allogeneic adipose stem cell group, each group, 20 rats in each group. The bone defect of 12 mm was constructed by partial resection of the middle part of ulna, and then the corresponding material was implanted. The bone mineral density was measured at the 12th week of the implant material. 14 weeks after the implantation, the solid fusion and the non-rigid fusion were observed in the three groups. Results and conclusion: the proportion of solid fusion was significantly higher in the group of scaffolds combined with allogeneic adipose stem cells than in the group of pure scaffold, and the proportion of non-rigid fusion and pseudarthrosis in the group of scaffolds combined with autogenous bone was significantly lower than that in the group of pure scaffold. The ratio of solid fusion in the pure scaffold group was significantly lower than that in the scaffold combined with autologous bone group. The ratio of unsturdy fusion and pseudarthrosis was significantly higher than that in the scaffold combined with autologous bone group (P 0.05). The bone mineral density (BMD) of the stents combined with allogeneic adipose stem cells (ASCs) was significantly higher than that of the stents alone at the 12th week after implantation. The results showed that the bone mineral density in the pure scaffold group was significantly lower than that in the scaffold combined with autologous bone group. The results showed that allograft adipose stem cells combined with nanometer collagenous bone material were effective in repairing ulnar bone defect and had higher bone density.
【作者单位】：中国中医科学院广安门医院骨科;北京中医药大学东直门医院推拿疼痛科;枣庄市薛城区中医院骨科;
【分类号】：R318.08;R687

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