海藻酸钙-纳米羟磷灰石骨修复材料的制备及部分生物相容性测定

发布时间：2018-03-13 16:01

本文选题：海藻酸钙　切入点：纳米羟磷灰石　出处：《华西口腔医学杂志》2014年01期 　论文类型：期刊论文

【摘要】：目的制备海藻酸钙-纳米羟磷灰石复合材料,探讨其作为骨修复材料的可行性。方法应用化学交联及冷冻干燥技术制备海藻酸钙-纳米羟磷灰石复合材料,通过扫描电镜(SEM)和X光衍射(XRD)对材料性能进行表征,液体置换法测定孔隙率。将第5代骨髓基质干细胞(BMSCs)接种至材料上,倒置显微镜及SEM观察细胞生长情况。材料浸提液与BMSCs共培养,甲基噻唑基四唑(MTT)法检测接种后1、3、5 d的相对增殖率(RGR),并评估毒性分级。新鲜犬血加入材料浸提液进行溶血试验,分光光度计测定光密度值并计算溶血率。结果制备的海藻酸钙-纳米羟磷灰石复合材料具有多孔性,孔隙率达(88.6±4.5)%。倒置显微镜及SEM观察可见,细胞在材料周围及表面伸展充分、生长旺盛。材料浸提液培养的细胞具有较好的活力,其毒性分级为1级。溶血试验结果表明复合材料的溶血率为1.28%,满足医用生物材料的要求。结论本研究制备的复合材料具有较高的孔隙率和良好的生物相容性。
[Abstract]:Objective to prepare calcium alginate-nano-hydroxyapatite composite and to explore its feasibility as bone repair material. Methods calcium alginate-nano-hydroxyapatite composite was prepared by chemical crosslinking and freeze-drying techniques. The properties of the materials were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The porosity was measured by liquid replacement method. The 5th generation bone marrow stromal cells (BMSCs) were inoculated onto the materials. The cell growth was observed by inverted microscope and SEM. The relative proliferative rate (RGRG) was measured by methylthiazolyl tetrazolium tetrazolium (MTT) method and the toxicity grade was evaluated. The hemolysis test was carried out by adding fresh canine blood into the material extract for hemolysis test, and the relative proliferation rate of RGRG was measured by methylthiazolyl tetrazolium (MTT) method at 5 days after inoculation. Results the prepared calcium alginate-nano-hydroxyapatite composite had porous properties, and the porosity was up to 88.6 卤4.50.The cells were well stretched around the material and on the surface of the composite by inverted microscope and SEM. The cells cultured in the material extract have good vitality. The results of hemolysis test show that the hemolysis rate of the composite is 1.28, which meets the requirements of medical biomaterials. Conclusion the composite prepared in this study has high porosity and good biocompatibility.
【作者单位】：安徽医科大学附属口腔医院颌面外科;安徽省口腔临床医学重点学科安徽省口腔疾病研究省级实验室中央与地方共建口腔医学中心实验室;安徽医科大学病原生物学教研室;
【基金】：安徽省自然科学基金资助项目(11040606M173) 安徽医科大学博士科研启动基金资助项目(XJ201034)
【分类号】：R318.08

【参考文献】