医用钛表面改性及其抗菌性和生物活性研究

发布时间：2018-03-13 19:32

本文选题：水热法　切入点：等离子体浸没离子注入　出处：《湖南大学》2012年博士论文　论文类型：学位论文

【摘要】：钛具有良好的生物相容性,被广泛用作硬组织替换材料。但是,钛是生物惰性材料,植入人体内易被当做异物。对医用钛进行表面改性,赋予医用钛理想的耐腐性能、生物活性和抗菌性,可有效改善其植入效果。本论文利用银/氮二元离子注入对钛进行表面改性,探查了银/氮二元离子注入顺序对钛表面的纳米硬度、耐腐能力和抗菌能力的影响。结合水热法和等离子体浸没离子注入技术,对医用钛进行表面改性,探查了改性钛表面的纳米结构、生物活性、抗菌性能和细胞相容性。此外,初步探讨了溶胶凝胶法制备聚乳酸(PLA)/CaO-Si02复合薄膜的生物活性和生物相容性。取得的主要研究成果如下： 1.利用等离子体浸没离子注入技术将银/氮二元离子按不同顺序(即先银后氮、先氮后银和银氮共注)注入钛的表面。银/氮二元离子注入钛表面的银以单质形式存在,而氮以氮化钛的形式存在。不同的银/氮二元注入顺序对改性后钛的耐腐性、纳米硬度和抗菌性能均有影响。银/氮二元离子共注钛表面的耐腐性能和硬度均优于先氮后银和先银后氮注入钛表面,这是因为银/氮二元共注导致的热效应使钛表面生成的氮化层最厚。银/氮二元注入样品对大肠杆菌和金黄色葡萄球菌均具有良好的抗菌作用。细胞实验表明,银/氮二元注入样品对细胞没有明显毒性,且有利于细胞的增殖和分化。 2.采用双氧水水热法在钛表面预先构建元素主要成份为Ti和O的不同纳米结构。利用等离子体浸没离子注入技术将银负载在双氧水水热处理钛表面,注银后样品表面纳米结构仍然保留完好。相对于纯钛片,注银前后钛基材的耐腐能力均获得了明显的改善。模拟体液浸泡实验显示,注银前后钛表面均具有良好的生物活性。注银样品对大肠杆菌和金黄色葡萄球菌繁殖具有明显抑制作用,显示出良好的抗菌性。细胞毒性实验表明,水热处理72小时钛表面注银前后对小鼠成骨细胞的繁殖和分化均具有良好的促进作用。 3.以氢氧化钠和双氧水作为反应介质,在钛表面构建了四种纳米结构,采用等离子体浸没离子注入技术将银负载在纳米结构表面。模拟体液浸泡实验显示,注银前后钛表面均显示良好的生物活性。注银表面对大肠杆菌和金黄色葡萄球菌均具有良好的抗菌能力。细胞毒性实验表明,除氢氧化钠水热处理并注银样品外,其它三种水热处理并注银钛表面对小鼠成骨细胞的增殖和分化具有较好的促进作用。 4.制备了不同比例的PLA/CaO-Si02多孔复合薄膜。在模拟体液浸泡7天的薄膜表面形成环状结构的类骨磷灰石层,显示良好生物活性。随着CaO-SiO2含量增加,复合薄膜表面微孔的孔径变小,薄膜表面zeta电位变负,诱导类骨磷灰石沉积的能力增强；MTT实验证实复合薄膜对MG-63细胞没有毒性且有利于细胞的增殖。
[Abstract]:Titanium has good biocompatibility and is widely used as a substitute for hard tissue. However, titanium is a biological inert material and is easily used as a foreign body in human body. In this paper, the surface modification of titanium was carried out by silver / nitrogen binary ion implantation, and the nano-hardness of titanium surface was investigated by silver / nitrogen binary ion implantation sequence. Combined with hydrothermal method and plasma immersion ion implantation technology, the surface modification of medical titanium was carried out, and the nanostructure, bioactivity, antibacterial property and cell compatibility of the modified titanium surface were investigated. The bioactivity and biocompatibility of poly (lactic acid) / CaO-Si02 composite films prepared by sol-gel method were preliminarily discussed. The main results were as follows:. 1. Silver / nitrogen binary ions were implanted into titanium surface by plasma immersion ion implantation in different order (that is, first silver then nitrogen, first nitrogen then silver and silver nitrogen co-implantation). However, nitrogen exists in the form of titanium nitride. Both nano-hardness and antimicrobial properties were affected. The corrosion resistance and hardness of Ag / N co-implanted titanium surface were better than that of Ag and Ag implanted titanium surface. This is because the thermal effect of silver / nitrogen binary injection results in the thickest nitrided layer on the surface of titanium. The silver / nitrogen binary implanted sample has good antibacterial effect on both Escherichia coli and Staphylococcus aureus. The silver / nitrogen binary implanted sample had no obvious toxicity to cells and was beneficial to cell proliferation and differentiation. 2. Different nanostructures with main elements Ti and O were pre-constructed on titanium surface by hydrogen peroxide hydrothermal method. Silver was loaded on titanium surface after heat treatment by plasma immersion ion implantation. The surface nanostructures of the samples remained intact after silver injection. Compared with pure titanium sheets, the corrosion resistance of titanium substrates before and after silver implantation was improved obviously. The simulated body fluid immersion test showed that the corrosion resistance of titanium substrates before and after silver injection was significantly improved. The surface of titanium has good biological activity before and after silver injection. The silver injected sample has obvious inhibitory effect on the reproduction of Escherichia coli and Staphylococcus aureus. After 72 hours of hydrothermal treatment, silver implantation on titanium surface could promote the proliferation and differentiation of mouse osteoblasts. 3. Using sodium hydroxide and hydrogen peroxide as the reaction medium, four kinds of nanostructures were constructed on the surface of titanium. Silver was loaded on the surface of the nanostructure by plasma immersion ion implantation technique. The surface of titanium showed good biological activity before and after silver injection, and the surface of silver injection had good antibacterial activity against Escherichia coli and Staphylococcus aureus. The cytotoxicity test showed that, except for sodium hydroxide hydrothermal treatment and silver injection, the surface of titanium had good antibacterial activity against Escherichia coli and Staphylococcus aureus. The other three kinds of hydrothermal treatment with silver and titanium can promote the proliferation and differentiation of mouse osteoblasts. 4. PLA/CaO-Si02 porous composite films with different proportions were prepared. The porous apatite layer with annular structure was formed on the surface of the membrane immersed in simulated body fluid for 7 days, which showed good biological activity. With the increase of CaO-SiO2 content, the pore size of micropores on the surface of the composite film became smaller. The zeta potential on the surface of the membrane was negative, and the ability to induce osteoid apatite deposition was enhanced. The results showed that the composite membrane was not toxic to MG-63 cells and was beneficial to cell proliferation.
【学位授予单位】：湖南大学
【学位级别】：博士
【学位授予年份】：2012
【分类号】：R318.08

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