脱细胞基质材料制备方法及在骨关节软骨损伤修复中的应用

发布时间：2018-03-14 04:24

本文选题：骨　切入点：软骨　出处：《中国组织工程研究》2016年34期 　论文类型：期刊论文

【摘要】：背景:研究显示,组织脱细胞基质与正常细胞外基质相比能够在机体内诱导上皮细胞、平滑肌细胞,促进其演化为机体的一部分。目的:观察脱细胞基质组织工程材料在骨关节软骨损伤修复中的应用效果。方法:采用随机对照方法将30只新西兰大白兔分为纤维样组织组和脱细胞基质组织组,每组15只,在大白兔股骨髁部建立直径为4 mm的骨关节软骨缺损模型。用牛膝关节软骨制备软骨脱细胞基质支架,脱细胞基质组织组采用脱细胞软骨基质支架修复;纤维样组织组采用纤维样组织修复,比较两组不同组织修复效果。结果与结论:(1)脱细胞基质组织工程材料交联后呈现为深蓝色,疏松多孔,直径为5 mm,硬度适中,具备一定的弹性;(2)苏木精-伊红染色不含有细胞碎屑及蓝染的核物质,不存在残留的细胞外基质;(3)甲苯胺蓝染色为蓝色材料支架孔隙率为90%,溶胀率为(1 314±337)%;(4)脱细胞基质组织组材料1,3,5,7,9 d的A值显著高于纤维样组织组(P0.05);(5)兔纤维样组织组缺损明显,纤维瘢痕基本形成并充填在缺损中。脱细胞基质组织组修复12周后缺损部位存在白色组织覆盖,表面光滑,缺损面基本修复,与正常组织边界不明显。甲苯甘蓝染色显示蓝染,细胞细小变平,软骨下骨骨结构与软骨连接良好,直接基本降解;(6)结果提示:脱细胞基质组织工程材料具有良好的生物相容性、细胞吸附性以及亲水性而在骨关节软骨损伤修复中得到应用,能促进损伤部位的修复效果,是一种适用于骨关节软骨损伤的修复材料。
[Abstract]:Background: studies have shown that the tissue acellular matrix can induce epithelial cells and smooth muscle cells in the organism compared with the normal extracellular matrix. Objective: to observe the application of acellular matrix tissue engineering materials in the repair of osteoarticular cartilage injury. Methods: 30 New Zealand white rabbits were divided into fibers by a randomized control method. Like tissue group and acellular matrix tissue group, The articular cartilage defect model with diameter of 4 mm was established in the femoral condyle of 15 rabbits in each group. Cartilage acellular matrix scaffold was prepared from Achyranthes bidentata articular cartilage, and acellular cartilage matrix scaffold was used to repair acellular cartilage matrix scaffold in acellular matrix tissue group. Results and conclusion the acellular matrix tissue engineering materials showed dark blue, porous, 5 mm diameter and moderate hardness after crosslinking. A certain amount of elasticity can be found in hematoxylin-eosin staining, which does not contain cytoclastic or blue-stained nuclear material. The porosity of the scaffold stained with toluidine blue was 90 and the swelling rate was 1 314 卤33 7) the A value of the acellular matrix tissue group was significantly higher than that of the fibrous tissue group (P 0. 05) and the defect of rabbit fibrous tissue group was significantly higher than that of the fibrous tissue group. The fibrous scar was basically formed and filled in the defect. After 12 weeks of acellular matrix tissue repair, the defect was covered with white tissue, the surface was smooth, the defect surface was basically repaired, and the boundary of the defect was not obvious with the normal tissue. Toluene cabbage staining showed blue staining. The results showed that the acellular matrix tissue engineering materials had good biocompatibility. Cell adsorption and hydrophilicity can be used in the repair of osteoarticular cartilage injury, which can promote the repair effect of the injured site. It is a suitable repair material for osteoarticular cartilage injury.
【作者单位】：南阳市中心医院;郑州大学第一附属医院;
【分类号】：R318.08;R684

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