硅酸盐生物活性玻璃与细菌吸附机制的研究

发布时间：2018-03-14 08:37

本文选题：45S5生物玻璃　切入点：细菌　出处：《华中师范大学》2012年硕士论文　论文类型：学位论文

【摘要】：目前对于硅酸盐生物玻璃的研究已经有了一定的进展,但是对于其抗菌作用的机制尚未形成系统的理论,尤其是对其与细菌吸附机制的研究目前在国内还没有文献系统报告过,相关文献表明,45S5抗菌原理为通过接触性破坏细菌的细胞壁,导致细菌死亡,是对组织细胞无损害的特异性杀菌机制。因此研究其吸附机制将可能成为其抗菌实际应用的关键理论基础,这对硅酸盐生物活性玻璃的研究应用有十分重大的意义,对与其它材料学及细胞或组织学的研究起到重要的参考作用。而本课题就是针对以上这些问题进行探讨,探究其抗菌机制下细菌吸附机制的作用模式,为抗菌课题进一步研究提供理论基础,对其实际应用提供理论支撑。本课题主要通过文献资料法、实验研究法、数理统计法观察并探究金黄色葡萄球菌在45S5生物活性玻璃表面的吸附现象,主要通过探究生物活性材料45S5对金葡菌的吸附机理以及金葡菌自身的吸附力的形成两方面来形成系统的理论。实验方法主要包括在体外采用细菌动态吸附实验,通过环境扫描电子显微镜、场发射电子显微镜等微观实验手段探究其动态变化,通过革兰氏染色、96孔板生物膜检测、ESS光密度曲线测定、等温吸附曲线测定、45S5的电荷吸附方式等实验手段探究生物玻璃与细菌吸附的本质机理。研究的主要结果如下： 1.45S5粉体对细菌有明显吸附作用,粒径小、浓度高的粉体吸附率较高。 2.45S5粉体与细菌的吸附过程是快速物理吸附过程,其吸附等温线可用Langmiur吸附方程描述。其吸附方式为典型的双电荷层吸附,电荷吸引力为其吸附的主要动力。45S5粉体能影响细菌自身生物膜的形成,使其被阻断于可逆性吸附阶段,细菌依靠吸附于粉体上的方式大多为细菌的沉积、Brown运动、细菌表面与蛋白吸附层(CB)之间的静电吸引、液体流动等非特异性的物理因素。
[Abstract]:At present, some progress has been made in the study of silicate bioglass, but the mechanism of its antibacterial action has not yet formed a systematic theory, especially the mechanism of its adsorption with bacteria has not been systematically reported in domestic literature. The related literature shows that the antibacterial principle of PER45S5 is to destroy the cell wall of bacteria by contact, resulting in the death of bacteria. It is a specific bactericidal mechanism without damage to tissue and cell. Therefore, the study of its adsorption mechanism may become the key theoretical basis for its practical application, which is of great significance to the research and application of silicate bioactive glass. It plays an important reference role in the study of other materials and cell or histology. This subject is to discuss the above problems and explore the mechanism of bacterial adsorption under the antibacterial mechanism. It provides a theoretical basis for further research on antimicrobial subject and theoretical support for its practical application. In this paper, the adsorption of Staphylococcus aureus on the surface of 45S5 bioactive glass was observed and explored by the methods of literature, experiment and statistics. The mechanism of adsorption of bioactive material 45S5 to Staphylococcus aureus and the formation of adsorption capacity of Staphylococcus aureus were studied to form a systematic theory. The dynamic changes of ESS were investigated by means of environmental scanning electron microscope and field emission electron microscope, and the optical density curve of ESS was detected by Gram staining and 96 hole biofilm. The isothermal adsorption curve was used to determine the charge-adsorption mode of 45S5. The main results were as follows: (1) the intrinsic mechanism of biosorption between bioglass and bacteria was investigated. 1.45S5 powder has obvious adsorption effect on bacteria, and the adsorption rate of the powder with small particle size and high concentration is higher. The adsorption process of 2.45S5 powder and bacteria is a rapid physical adsorption process, and its adsorption isotherm can be described by Langmiur adsorption equation. The charge attraction is the main force of adsorption. 45S5 powder can affect the formation of bacterial biofilm and block it at the reversible adsorption stage. Non-specific physical factors such as electrostatic attraction and liquid flow between bacterial surface and protein adsorption layer (CBB).
【学位授予单位】：华中师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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