低聚乙二醇富马酸酯水凝胶中骨髓来源间充质干细胞的生物学行为

发布时间：2018-03-17 13:46

本文选题：凝胶类　切入点：间质干细胞　出处：《中国组织工程研究》2016年16期 　论文类型：期刊论文

【摘要】：背景:低聚乙二醇富马酸酯水凝胶是一种具有良好生物相容性及可注射性和可降解性的生物材料。不同相对分子质量水凝胶之间的特性有所差异,将骨髓间充质干细胞包裹其中并诱导细胞成骨分化,相对分子质量相当的水凝胶更有利于细胞增殖和分化,所以采用该材料为骨组织工程支架提供了新的选择。目的:探讨不同相对分子质量的低聚乙二醇富马酸酯水凝胶材料体外包裹大鼠骨髓间充质干细胞的增殖和分化的影响。方法:低聚乙二醇富马酸酯通过氧化还原基团引发系统产生交联,制备出相对分子质量为1 000,3 000,10 000,35 000的低聚乙二醇富马酸酯水凝胶,对水凝胶的溶胀和降解性能进行检测。将骨髓间充质干细胞包裹到4种相对分子质量的水凝胶中,在成骨培养液中诱导1-3周,通过组织学染色(苏木精-伊红染色和茜素红染色)和免疫荧光染色检测水凝胶材料对骨髓间充质干细胞形态的影响以及成骨分化的效果。结果与结论:①随着水凝胶相对分子质量的增加,成胶时间变短,凝胶的溶胀度明显增加,且随着时间的推移,水凝胶的降解速率与相对分子质量成正比;②细胞复合水凝胶支架材料的组织学与免疫荧光染色结果表明,细胞经过诱导后,在具有适当溶胀与降解特性的相对分子质量为3 000与10 000的水凝胶中所形成的矿化结节数量显著多于在其它两种相对分子质量中的,说明有利于细胞的增殖与分化;③结果表明,低聚乙二醇富马酸酯水凝胶具有良好的生物相容性,且相对分子质量为3 000与10 000的水凝胶对间充质干细胞的成骨分化有一定的良性调节作用。
[Abstract]:Background: polyethylene glycol fumarate hydrogel is a biocompatible, injectable and degradable biomaterial. Bone marrow mesenchymal stem cells were encapsulated and osteoblast differentiation was induced. The hydrogel with the same molecular weight was more favorable for cell proliferation and differentiation. Therefore, this material provides a new choice for bone tissue engineering scaffold. Objective: to investigate the proliferation and differentiation of rat bone marrow mesenchymal stem cells coated with different molecular weight polyethylene glycol fumarate hydrogel materials in vitro. Methods: polyethylene glycol fumarate produced crosslinking by redox group initiation system. Polyethylene glycol fumarate hydrogels with relative molecular weight of 1 000 ~ 3 000 ~ 10 000 ~ 35 000 were prepared, and the swelling and degradation properties of hydrogels were tested. Bone marrow mesenchymal stem cells were encapsulated into four hydrogels with relative molecular weight. 1 to 3 weeks after induction in osteoblasts. Histological staining (hematoxylin-eosin staining and alizarin red staining) and immunofluorescence staining were used to detect the effect of hydrogel material on morphology and osteogenic differentiation of bone marrow mesenchymal stem cells. The increase in the relative molecular weight of the gel, The gelation time became shorter, the swelling degree of the gel increased obviously, and with the passage of time, the degradation rate of hydrogel was directly proportional to the relative molecular weight of the hydrogel scaffold. The histological and immunofluorescence staining results of the hydrogel scaffold material were as follows:. After induction, the number of mineralized nodules formed in hydrogels with proper swelling and degradation characteristics of 3 000 and 10 000 molecular weight was significantly higher than that in the other two kinds of relative molecular weight. The results showed that the hydrogels of polyethylene glycol fumarate had good biocompatibility. The hydrogels with relative molecular weight of 3 000 and 10 000 have a positive effect on the osteogenic differentiation of mesenchymal stem cells.
【作者单位】：解放军总医院口腔科;军事医学科学院基础医学研究所;
【基金】：国家自然科学基金(31271054)~~
【分类号】：R318.08

【相似文献】